Advertisement

Bulletin of Experimental Biology and Medicine

, Volume 168, Issue 3, pp 352–355 | Cite as

Semaphorin-3A Inhibits Proliferation, but Does Not Affect Apoptosis of Mouse Thymocytes In Vitro

  • K. V. RuttoEmail author
  • V. I. Lyudyno
  • I. V. Kudryavtsev
  • E. P. Kiseleva
MICROBIOLOGY AND IMMUNOLOGY
  • 3 Downloads

Neuronal factor semaphorin-3A is viewed as immune suppressant of peripheral T lymphocytes, but it can also negatively affect activity of the thymus, the central organ of the immune system. The study examined the effects of this factor on proliferative activity and apoptosis of mouse thymocytes in vitro. Semaphorin-3A inhibited spontaneous and mitogen-stimulated proliferative activity of thymocytes producing no effect on the development of apoptosis in these cells. Flow cytometry revealed expression of semaphorin-3A receptors neuropilin-1 and plexin-A1 on thymocyte membranes. Approximately 13% thymocytes simultaneously expressed both receptors. The study suggests that semaphorin-3A, which is constitutively synthesized in thymic stroma in vivo, can play the role of inhibitory factor during thymocyte maturation.

Key Words

semaphorin-3A thymocytes proliferation neuropilin-1 plexin-A1 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Kisseleva EP, Lyamina IV, Tzvirkun SA, Rutto KV, Kudryavtsev IV, Lioudyno VI. Investigation of the role of semaphorin 3A in the thymus at normal conditions and tumor growth. Med. Akad. Zh. 2013;13(4):42-48. Russian.Google Scholar
  2. 2.
    Rutto KV, Ovsyukov KS, Kudryavtsev IV, Kiseleva EP. Semaphorin 3A Negatively Affects Proliferation of Mouse Thymus Epithelial Cells In Vitro. Bull. Exp. Biol. Med. 2019;166(3): 339-343. doi:  https://doi.org/10.1007/s10517-019-04346-x CrossRefPubMedGoogle Scholar
  3. 3.
    Catalano A, Caprari P, Moretti S, Faronato M, Tamagnone L, Procopio A. Semaphorin-3A is expressed by tumor cells and alters T-cell signal transduction. Blood. 2006;107(8):3321-3329.CrossRefGoogle Scholar
  4. 4.
    Corbel C, Lemarchandel V, Thomas-Vaslin V, Pelus AS, Agboton C, Roméo PH. Neuropilin 1 and CD25 co-regulation during early murine thymic differentiation. Dev. Comp. Immunol. 2007;31(11):1082-1094.CrossRefGoogle Scholar
  5. 5.
    Garcia F, Lepelletier Y, Smaniotto S, Hadj-Slimane R, Dardenne M, Hermine O, Savino W. Inhibitory effect of semaphorin-3A, a known axon guidance molecule in the human thymocyte migration induced by CXCL12. J. Leukoc. Biol. 2012;91(1):7-13.CrossRefGoogle Scholar
  6. 6.
    Hansen MB, Nielsen SE, Berg K. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J. Immunol. Methods. 1989;119(2):203-210.CrossRefGoogle Scholar
  7. 7.
    Ji JD, Park-Min KH, Ivashkiv LB. Expression and function of semaphorin 3A and its receptors in human monocyte-derived macrophages. Hum. Immunol. 2009;70(4):211-217.CrossRefGoogle Scholar
  8. 8.
    Lepelletier Y, Moura IC, Hadj-Slimane R, Renand A, Fiorentino S, Baude C, Shirvan A, Barzilai A, Hermine O. Immunosuppressive role of semaphorin-3A on T cell proliferation is mediated by inhibition of actin cytoskeleton reorganization. Eur. J. Immunol. 2006;36(7):1782-1793.CrossRefGoogle Scholar
  9. 9.
    Lepelletier Y, Smaniotto S, Hadj-Slimane R, Villa-Verde DM, Nogueira AC, Dardenne M, Hermine O, Savino W. Control of human thymocyte migration by Neuropilin-1/Semaphorin-3A-mediated interactions. Proc. Natl Acad. Sci. USA. 2007;104(13):5545-5550.CrossRefGoogle Scholar
  10. 10.
    Lins MP, Silva ECO, Silva GR, Souza ST, Medeiros NC, Fonseca EJS, Smaniotto S. Association between biomechanical alterations and migratory ability of semaphorin-3Atreated thymocytes. Biochim. Biophys. Acta. Gen. Subj. 2018;1862(4):816-824.CrossRefGoogle Scholar
  11. 11.
    Mendes-da-Cruz DA, Lepelletier Y, Brignier AC, Smaniotto S, Renand A, Milpied P, Dardenne M, Hermine O, Savino W. Neuropilins, semaphorins, and their role in thymocyte development. Ann. NY Acad. Sci. 2009;1153:20-28.CrossRefGoogle Scholar
  12. 12.
    Moretti S, Procopio A, Lazzarini R, Rippo MR, Testa R, Marra M, Tamagnone L, Catalano A. Semaphorin3A signaling controls Fas (CD95)-mediated apoptosis by promoting Fas translocation into lipid rafts. Blood. 2008;111(4):2290-2299.CrossRefGoogle Scholar
  13. 13.
    Stemberger J, Witt V, Printz D, Geyeregger R, Fritsch G. Novel single-platform multiparameter FCM analysis of apoptosis: significant differences between wash and no-wash procedure. Cytometry A. 2010;77(11):1075-1081.CrossRefGoogle Scholar
  14. 14.
    Takamatsu H, Kumanogoh A. Diverse roles for semaphorinplexin signaling in the immune system. Trends Immunol. 2012;33(3):127-135.CrossRefGoogle Scholar
  15. 15.
    Vadasz Z, Toubi E. Semaphorin 3A: a potential therapeutic tool in immune-mediated diseases. Eur. J. Rheumatol. 2018;5(1):58-61.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • K. V. Rutto
    • 1
    Email author
  • V. I. Lyudyno
    • 1
  • I. V. Kudryavtsev
    • 1
  • E. P. Kiseleva
    • 1
    • 2
  1. 1.Institute of Experimental MedicineSt. PetersburgRussia
  2. 2.I. I. Mechnikov North-Western State Medical UniversitySt. PetersburgRussia

Personalised recommendations