Bulletin of Experimental Biology and Medicine

, Volume 168, Issue 3, pp 352–355 | Cite as

Semaphorin-3A Inhibits Proliferation, but Does Not Affect Apoptosis of Mouse Thymocytes In Vitro

  • K. V. RuttoEmail author
  • V. I. Lyudyno
  • I. V. Kudryavtsev
  • E. P. Kiseleva

Neuronal factor semaphorin-3A is viewed as immune suppressant of peripheral T lymphocytes, but it can also negatively affect activity of the thymus, the central organ of the immune system. The study examined the effects of this factor on proliferative activity and apoptosis of mouse thymocytes in vitro. Semaphorin-3A inhibited spontaneous and mitogen-stimulated proliferative activity of thymocytes producing no effect on the development of apoptosis in these cells. Flow cytometry revealed expression of semaphorin-3A receptors neuropilin-1 and plexin-A1 on thymocyte membranes. Approximately 13% thymocytes simultaneously expressed both receptors. The study suggests that semaphorin-3A, which is constitutively synthesized in thymic stroma in vivo, can play the role of inhibitory factor during thymocyte maturation.

Key Words

semaphorin-3A thymocytes proliferation neuropilin-1 plexin-A1 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • K. V. Rutto
    • 1
    Email author
  • V. I. Lyudyno
    • 1
  • I. V. Kudryavtsev
    • 1
  • E. P. Kiseleva
    • 1
    • 2
  1. 1.Institute of Experimental MedicineSt. PetersburgRussia
  2. 2.I. I. Mechnikov North-Western State Medical UniversitySt. PetersburgRussia

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