Bulletin of Experimental Biology and Medicine

, Volume 168, Issue 2, pp 270–274 | Cite as

Detection of Small Subsets of CD4+ Lymphocytes with SmartFlare Nanoprobes

  • A. P. ToptyginaEmail author
  • R. Sh. Zakirov
  • K. S. Kapitanova
  • E. L. Semikina

SmartFlare technology allows detection of mRNA in single living cells. We studied the possibility of using SmartFlare nanoprobes for detection of small subsets of CD4+ lymphocytes. It was found that SmartFlare allows detection of transcriptional master regulators of major CD4+T helper subsets in living human lymphocytes. Nanoprobes labeled with Cy5 fluorophore were better detected by flow cytometry than nanoprobes labeled with Cy3. Appropriate time of lymphocyte incubation with SmartFlare probes was 24 h.

Key Words

subsets of CD4+ lymphocytes SmartFlare transcriptional factors 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • A. P. Toptygina
    • 1
    Email author
  • R. Sh. Zakirov
    • 2
  • K. S. Kapitanova
    • 1
  • E. L. Semikina
    • 2
  1. 1.G. N. Gabrichevsky Moscow Research Institute for Epidemiology and MicrobiologyFederal Service on Surveillance for Consumer Rights Protection and Human Well-BeingMoscowRussia
  2. 2.National Medical Research Center for Children’s HealthMinistry of Health of the Russian FederationMoscowRussia

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