Bulletin of Experimental Biology and Medicine

, Volume 168, Issue 2, pp 259–263 | Cite as

Effect of Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide on Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis

  • L. B. KimEmail author
  • A. N. Putyatina
  • G. S. Russkikh
  • V. A. Shkurupy

Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix. Both dextrazide and its liposomal form decreased the levels of sulfated glycosaminoglycans and uronic acids. In contrast to INH, both preparations did not decrease the levels of total glycosaminoglycans, proteins, and galactose. This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.

Key Words

liposomal dextrazide isonicotinic acid hydrazide BCG-induced granulomatosis extracellular matrix mouse lungs 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • L. B. Kim
    • 1
    Email author
  • A. N. Putyatina
    • 1
  • G. S. Russkikh
    • 1
  • V. A. Shkurupy
    • 1
    • 2
  1. 1.Federal Research Center of Fundamental and Translational MedicineNovosibirskRussia
  2. 2.Novosibirsk State Medical UniversityMinistry of Health of the Russian FederationNovosibirskRussia

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