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Bulletin of Experimental Biology and Medicine

, Volume 168, Issue 2, pp 187–192 | Cite as

Gadolinium as an Inhibitor of Ionic Currents in Isolated Rat Ventricular Cardiomyocytes

  • A. L. Shim
  • A. G. KamkinEmail author
  • O. V. Kamkina
  • V. E. Kazanskii
  • V. M. Mitrokhin
  • A. S. Bilichenko
  • T. S. Filatova
  • D. V. Abramochkin
PHYSIOLOGY
  • 6 Downloads

The whole-cell patch-clamp technique was used to examine the effect of gadolinium Gd3+ (a non-specific blocker of mechanically gated current IMGCh, a component of late current IL) on ionic currents in insolated rat ventricular cardiomyocytes alone and in combination with the blockers of L-type calcium currents (ICaL) nifedipine (10 μM) or verapamil (1 μM). In K+in/K+out or Cs+in/Cs+out media, blockade of ICaL produced no effect on IL at negative potentials, but inhibited IL at positive ones. In K+in/K+out medium, Gd3+ (5 μM) decreased the net persistent current (Inp) at -45 mV from 198.6±6.4 to 96.7±9.5 pA over 15 min. Gd3+ alone or in combination with ICaL blockers shifted the reversal potential of IL to more negative values. At negative potentials, Gd3+ decreased IK1 and inward current including IMGCh. At positive potentials, Gd3+ alone or in combination with ICaL blockers decreased IL. When applied for 15 min in Cs+in/Cs+out medium at -45 mV, Gd3+ produced no effect on net current and inward and outward components of IL. Thus, Gd3+ can be viewed as a specific blocker of IMGCh only in Cs+ medium.

Key Words

rat isolated cardiomyocytes gadolinium patch-clamp ionic currents 

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • A. L. Shim
    • 1
  • A. G. Kamkin
    • 1
    Email author
  • O. V. Kamkina
    • 1
  • V. E. Kazanskii
    • 1
  • V. M. Mitrokhin
    • 1
  • A. S. Bilichenko
    • 1
  • T. S. Filatova
    • 1
    • 2
  • D. V. Abramochkin
    • 1
    • 2
  1. 1.N. I. Pirogov Russian National Research Medical UniversityMinistry of Health of the Russian FederationMoscowRussia
  2. 2.Faculty of BiologyM. V. Lomonosov Moscow State UniversityMoscowRussia

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