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Bulletin of Experimental Biology and Medicine

, Volume 168, Issue 1, pp 145–149 | Cite as

Content of Free Fetal DNA in Maternal Blood and Expression of DNA Recognition Receptors ZBP-1 in Placental Tissue in Preeclampsia and Preterm Labor

  • O. R. Baev
  • A. O. Karapetian
  • N. V. NizyaevaEmail author
  • А. А. Sadekova
  • A. M. Krasniy
Article

We evaluated the content of cell-free fetal DNA in maternal blood and expression of ZBP-1 receptors in the placental tissue of women with uncomplicated pregnancy, preeclampsia, and preterm labor. The study included 16 women with preeclampsia (early and late-onset preeclampsia, 8 cases each), 16 women with preterm labor, and 21 women with uncomplicated pregnancy. The concentration of cell-free fetal DNA was measured by PCR by detecting hypermethylated region of the RASSF1A gene. Immunohistochemistry was performed on paraffin-embedded sections of the placenta samples using primary polyclonal antibodies to ZBP-1. Significant increase in the level of cell-free fetal DNA was found in women with preeclampsia (both early and late-onset form) in comparison with uncomplicated pregnancy. The concentration of cell-free fetal DNA in preterm labor group did not differ from the control group; however, it was significantly lower than in early-onset preeclampsia, but not late preeclampsia. Immunohistochemical study showed higher expression of ZBP-1 in the villus syncytiotrophoblast in early-onset preeclampsia in comparison with that in preterm labor group (p=0.006). Fragments of damaged placental cells, predominantly trophoblast, enter maternal circulation and are the source of cell-free fetal DNA and a potential ligand for ZBP-1, which leads to further cell damage and the formation of a vicious circle. The increase in the content of cell-free fetal DNA in maternal blood and ZBP-1 expression in the syncytiotrophoblast in preeclampsia are interrelated processes reflecting impaired morphofunctional state of the placenta.

Key Words

cell-free fetal DNA RASSF1A gene ZBP-1 preeclampsia preterm labor 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • O. R. Baev
    • 1
    • 2
  • A. O. Karapetian
    • 1
  • N. V. Nizyaeva
    • 1
    Email author
  • А. А. Sadekova
    • 1
  • A. M. Krasniy
    • 1
    • 3
  1. 1.V. I. Kulakov National Research Center for Obstetrics, Gynecology, and PerinatologyMinistry of Health of Russian FederationMoscowRussia
  2. 2.Department of Obstetrics, Gynecology, Perinatology, and ReproductologyI. M. Sechenov First Moscow State Medical University, Ministry of Health of Russian Federation (Sechenov University)MoscowRussia
  3. 3.N. K. Koltsov Institute of Developmental BiologyRussian Academy of SciencesMoscowRussia

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