Bulletin of Experimental Biology and Medicine

, Volume 168, Issue 1, pp 125–131 | Cite as

Phenotype and Secretome of Monocyte-Derived Macrophages Interacting with Mesenchymal Stromal Cells under Conditions of Hypoxic Stress

  • O. Yu. Alekseeva
  • P. I. Bobyleva
  • E. R. AndreevaEmail author
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)

We studied the effect of short-term hypoxic stress on the phenotypic polarization of monocyte-derived macrophages and their secretory activity during interaction with mesenchymal stromal cells. In the presence of mesenchymal stromal cells, monocyte-derived macrophages exhibited the signs of M2 polarization, which was evidenced by increased expression of CD206 and CD163 markers, as well as increased transcription and translation of IL-6. Short-term hypoxic stress promoted a shift of macrophage phenotype from inflammatory M1 towards anti-inflammatory M2 in monoculture and co-culture with mesenchymal stromal cells. In addition to the immunoregulatory action, mesenchymal stromal cells demonstrated stromal activity and maintained high viability of monocyte-derived macrophages.

Key Words

monocytes/macrophages multipotent mesenchymal stromal cells hypoxia phenotype polarization secretome 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • O. Yu. Alekseeva
    • 1
  • P. I. Bobyleva
    • 1
  • E. R. Andreeva
    • 1
    Email author
  1. 1.State Research Center Institute of Biomedical ProblemsRussian Academy of SciencesMoscowRussia

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