Advertisement

Bulletin of Experimental Biology and Medicine

, Volume 167, Issue 6, pp 813–817 | Cite as

Morphofunctional Differences of Micronuclei in Cultures of Human p53-Positive and p53-Negative Tumor Cells

  • O. I. SutyaginaEmail author
  • O. P. Kisurina-Evgenieva
Article
  • 32 Downloads

Micronuclei that are often present in tumor cells are not only the indicator of genetic instability, they also can induce DNA damage during cell progression through the cell cycle. p53 protein is the key regulator of the cell cycle. In this study we compared morphofunctional features of micronuclei depending on the presence of wild-type p53 gene: in human breast adenocarcinoma MCF-7 (p53+) and human epidermoid carcinoma A431 (p53). The number of cells with MN in these cell lines does not depend on the presence of active p53. However, micronuclei in cell culture with mutant p53 protein more often have lamina defects, carry DNA damage, and generally determine higher risk of tumor progression. Asynchronous with the main nucleus DNA replication in micronuclei in p53+ and p53 cell cultures demonstrates predisposition of cells with micronuclei to chromothripsis.

Key Words

micronuclei nuclear lamina γН2Аx p53 replication 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Vildanova MS, Savitskaya MA, Onishchenko GE, Smirnova EA. Effect of plant hormones on the components of secretory pathway in human normal and tumor cells. Tsitologiya. 2014;56(7):516-525. Russian.Google Scholar
  2. 2.
    Kisurina-Evgen’eva OP, Bryantseva SA, Onishchenko GE, Shtil’ AA. Antitubulin agents can initiate different apoptotic pathways. Biophysics. 2006;51(5):771-775.CrossRefGoogle Scholar
  3. 3.
    Savitskaya MA, Vildanova MS, Kisurina-Evgenieva OP, Smirnova EA, Onischenko GE. Mitochondrial pathway of α-tocopheryl succinate-induced apoptosis in human epidermoid carcinoma A431 cells. Acta Naturae. 2012;4(3):88-94.CrossRefGoogle Scholar
  4. 4.
    Chumakov PM. Protein p53 and its universal functions in multicellular organism. Uspekhi Biol. Khimii. 2007;47(1):3-52. Russian.Google Scholar
  5. 5.
    Asare N, Instanes C, Sandberg WJ, Refsnes M, Schwarze P, Kruszewski M, Brunborg G. Cytotoxic and genotoxic effects of silver nanoparticles in testicular cells. Toxicology. 2012;291(1-3):65-72.CrossRefGoogle Scholar
  6. 6.
    Crasta K, Ganem NJ, Dagher R, Lantermann AB, Ivanova EV, Pan Y, Nezi L, Protopopov A, Chowdhury D, Pellman D. DNA breaks and chromosome pulverization from errors in mitosis. Nature. 2012;482:53-58.CrossRefGoogle Scholar
  7. 7.
    Fenech M, Kirsch-Volders M, Natarajan AT, Surralles J, Crott JW, Parry J, Norppa H, Eastmond DA, Tucker JD, Thomas P. Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells. Mutagenesis. 2011;26(1):125-132.CrossRefGoogle Scholar
  8. 8.
    Hatch EM, Fischer AH, Deerinck TJ, Hetzer MW. Catastrophic nuclear envelope collapse in cancer cell micronuclei. Cell. 2013;154(1):47-60.CrossRefGoogle Scholar
  9. 9.
    Ly P, Cleveland DW. Rebuilding chromosomes after catastrophe: emerging mechanisms of chromothripsis. Trends Cell Biol. 2017;27(12):917-930.CrossRefGoogle Scholar
  10. 10.
    Morrison AJ, Shen X. DNA repair in the context of chromatin. Cell Cycle. 2005;4(4):568-571.CrossRefGoogle Scholar
  11. 11.
    Park DJ, Nakamura H, Chumakov AM, Said JW, Miller CW, Chen DL, Koeffler HP. Transactivational and DNA binding abilities of endogenous p53 in p53 mutant cell lines. Oncogene. 1994;9(7):1899-1906.PubMedGoogle Scholar
  12. 12.
    Sablina AA, Ilyinskaya GV, Rubtsova SN, Agapova LS, Chumakov PM, Kopnin BP. Activation of p53-mediated cell cycle checkpoint in response to micronuclei formation. Сell. 1998;111(Pt 7):977-984.Google Scholar
  13. 13.
    Zhang CZ, Spektor A, Cornils H, Francis JM, Jackson EK, Liu S, Meyerson M, Pellman D. Chromothripsis from DNA damage in micronuclei. Nature. 2015;522:179-184.CrossRefGoogle Scholar
  14. 14.
    Zuela N, Bar DZ, Gruenbaum Y. Lamins in development, tissue maintenance and stress. EMBO Rep. 2012;13(12):1070-1078.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Cell Biology and Histology, Faculty of BiologyM. V. Lomonosov Moscow State UniversityMoscowRussia

Personalised recommendations