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Bulletin of Experimental Biology and Medicine

, Volume 167, Issue 6, pp 801–804 | Cite as

Histotopography of α-Gustdusin- and T2R3-Expressing Lymphocytes in Mouse Spleen

  • I. A. Cherenkov
  • V. G. SergeyevEmail author
  • D. B. Nikityuk
  • V. M. Chychkov
Article
  • 32 Downloads

Histotopography of lymphocytes expressing bitter taste receptors of T2R family and α-gustducin (receptor-associated subunit of the G-protein complex) in mouse spleen in the norm and in 48 h after intraperitoneal administration of LPS was studied by the immunohistochemical method. Two populations of immunopositive lymphocytes expressing the above proteins were detected in the spleen; they were located in the marginal zones of lymphoid follicles and periarteriolar lymphoid sheaths. In most α-gustducin-positive lymphocytes, co-expression of α-gustducin and CD19 (B-cell marker) was found. Intraperitoneal administration of LPS significantly increased the number of gustducinergic lymphocytes in periarteriolar lymphoid sheaths. We hypothesize that spleen lymphocytes with gustducin signaling are involved in T-dependent immune response to the blood bacterial polysaccharides.

Key Words

T2R3 receptor α-gustducin spleen lymphocytes 

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References

  1. 1.
    Semenova MA, Zakolyukina ES, Sergeev VG. Expression of α-gustducin in oral and nasopharyngeal lympocytes and cheosensory cells. Vestn. Udmurt. Univer. Ser. Biologiya. Nauki Zemle. 2016;26(1):133-139. Russian.Google Scholar
  2. 2.
    Breslin PA. An evolutionary perspective on food and human taste. Curr. Biol. 2013;23(9):R409-R418.CrossRefGoogle Scholar
  3. 3.
    Cariappa A, Tang M, Parng C, Nebelitskiy E, Carroll M, Georgopoulos K, Pillai S. The follicular versus marginal zone B lymphocyte cell fate decision is regulated by Aiolos, Btk, and CD21. Immunity 2001;14(5):603-615.CrossRefGoogle Scholar
  4. 4.
    Kehrl JH. G-protein-coupled receptor signaling, RGS proteins, and lymphocyte function. Crit. Rev. Immunol. 2004;24(6):409-423.CrossRefGoogle Scholar
  5. 5.
    Malki A, Fiedler J, Fricke K, Ballweg I, Pfaffl MW, Krautwurst D. Class I odorant receptors, TAS1R and TAS2R taste receptors, are markers for subpopulations of circulating leukocytes. Leukoc. Biol. 2015;97(3):533-545.CrossRefGoogle Scholar
  6. 6.
    Margolskee RF. Molecular mechanisms of bitter and sweet taste transduction. J. Biol. Chem. 2002;277(1):1-4.CrossRefGoogle Scholar
  7. 7.
    Martin F, Oliver AM, Kearney JF. Marginal zone and B1 B cells unite in the early response against T-independent blood-borne particulate antigens. Immunity. 2001;14(5):617-629.CrossRefGoogle Scholar
  8. 8.
    Mebius RE, Kraal G. Structure and function of the spleen. Nat. Rev. Immunol. 2005;5(8):606-616.CrossRefGoogle Scholar
  9. 9.
    Takeda K, Akira S. Roles of Toll-like receptors in innate immune responses. Genes Cells 2001;6(9):733-742.CrossRefGoogle Scholar
  10. 10.
    Verbeurgt C, Veithen A, Carlot S, Tarabichi M, Dumont JE, Hassid S, Chatelain P. The human bitter taste receptor T2R38 is broadly tuned for bacterial compounds. PLoS One. 2017;12(9). ID e0181302. doi:  https://doi.org/10.1371/journal.pone.0181302 CrossRefGoogle Scholar
  11. 11.
    Zhao W, Ho L, Varghese M, Yemul S, Dams-O’Connor K, Gordon W, Knable L, Freire D, Haroutunian V, Pasinetti GM. Decreased level of olfactory receptors in blood cells following traumatic brain injury and potential association with tauopathy. J. Alzheimers Dis. 2013;34(2):417-429.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • I. A. Cherenkov
    • 1
  • V. G. Sergeyev
    • 1
    Email author
  • D. B. Nikityuk
    • 2
  • V. M. Chychkov
    • 1
  1. 1.Udmurt State UniversityIzhevskRussia
  2. 2.Federal Research Center of Nutrition, Biotechnology, and Food SafetyMoscowRussia

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