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Bulletin of Experimental Biology and Medicine

, Volume 167, Issue 5, pp 676–680 | Cite as

Gas Chromatography–Mass Spectrometry Analysis of Urinary Steroid Metabolomics for Detection of Early Signs of Adrenal Neoplasm Malignancy in Patients with Cushing’s Syndrome

  • L. I. VelikanovaEmail author
  • Z. R. Shafigullina
  • N. V. Vorokhobina
  • E. V. Malevanaya
Article
  • 11 Downloads

The metabolomics of urinary steroids was studied by gas chromatography-mass spectrometry in 25 patients with Cushing’s syndrome without malignant potential and in 12 patients with malignant potential of adrenal neoplasms (Weiss score 1-3). Patients with adrenocortical adenoma (N=24) constituted the control group. In patients with Cushing’s syndrome and malignant potential, increased urinary excretion of 16-oxo-androstendiol, tetrahydro-11-deoxycortisol, and 16-hydroxypregnendiol, which had 100% specificity and sensitivity >90% for the diagnosis of malignant potential. Additionally, non-classical 5-ene-pregnenes (16-OHpregnenolone, 21-OH-pregnenolone, 3β,16,20-pregnentriol, and 3β,17,20-pregnentriol) were identified. The revealed changes in the metabolomics of steroids can be early signs of malignant potential in patients with Cushing’s syndrome. In patients with malignant potential, three signs of reduced activity of 11β-hydroxysteroid dehydrogenase type 2 were detected and in patients without malignant potential, one sign was found. In patients with and without malignant potential, three signs increased activity of 5β-reductase were found.

Key Words

steroid profile in urine gas chromatography-mass spectrometry Cushing’s syndrome malignant potential 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • L. I. Velikanova
    • 1
    Email author
  • Z. R. Shafigullina
    • 1
  • N. V. Vorokhobina
    • 1
  • E. V. Malevanaya
    • 1
  1. 1.I. I. Mechnikov North-Western State Medical University, Ministry of Health of the Russian FederationSt. PetersburgRussia

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