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Bulletin of Experimental Biology and Medicine

, Volume 167, Issue 5, pp 628–633 | Cite as

High-Carbohydrate Diets Affect Accumulation of Lipofuscin-Like Pigment in the Kidneys of Mice and Rats: Autofluorescence Confocal Microscopy Analysis

  • S. A. Apryatin
  • M. O. Semin
  • I. V. GmoshinskiiEmail author
  • D. B. Nikityuk
BIOPHYSICS AND BIOCHEMISTRY
  • 20 Downloads

The accumulation of lipofuscin-like granules in liver, kidneys, and spleen cells in mice and rats of different lines receiving 30% sugar solutions (fructose, glucose, their mixture, and sucrose) in addition to balanced semisynthetic diet for 62 or 122 days was studied by the method of laser scanning confocal microscopy. The granules were detected by their autofluorescence at maximum λex =570-600 nm and λex=488 nm. In the kidneys of rats receiving glucose and, especially, the mixture of glucose and fructose, significant accumulation of lipofuscin-like granules was found that was absent in the control group animals receiving water. Intensive accumulation of the granules was observed in the kidneys of all groups of mice receiving sugars (except for glucose). Lipofuscin-like granules were located in the cytoplasm of epithelial cells of the distal and proximal convoluted tubules. In the liver of rats and mice, the signs of accumulation of lipofuscin-like granules were absent or minimal. In rat spleen, lipofuscinlike granules were found in the red pulp in all groups, but their accumulation significantly increased in animals receiving the diet enriched with glucose and sucrose.

Key Words

high-carbohydrate diets aging lipofuscin rats confocal microscopy 

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References

  1. 1.
    Efimov AA, Maslyakova GN. Lipofuscin role in involutive and pathological processes. Saratov. Nauch.-Med. Zh. 2009;5(1):111-115. Russian.Google Scholar
  2. 2.
    Moulisová V, Srbová M, Jedlicková O, Sebestian J, Jegorov A. Silybin reduces lipid peroxidation of rat hepatocyte membrane caused by cyclosporin A. Biochemistry (Mosc). 2006;71(10):1110-1114.CrossRefGoogle Scholar
  3. 3.
    Brunk UT, Terman A. The mitochondrial-lysosomal axis theory of aging: accumulation of damaged mitochondria as a result of imperfect autophagocytosis. Eur. J. Biochem. 2002;269(8):1996-2002.CrossRefGoogle Scholar
  4. 4.
    Castrejón-Tellez V, Rodríguez-Pérez JM, Pérez-Torres I, Pérez-Hernández N, Cruz-Lagunas A, Guarner-Lans V, Vargas-Alarcón G, Rubio-Ruiz ME. The effect of resveratrol and quercetin treatment on PPAR mediated uncoupling protein (UCP-) 1, 2, and 3 expression in visceral white adipose tissue from metabolic syndrome rats. Int. J. Mol. Sci. 2016;17(7). pii: E1069. doi: 10.3390/ijms17071069.CrossRefGoogle Scholar
  5. 5.
    Csallany AS, Ayaz KL, Su LC. Effect of dietary vitamin E and aging on tissue lipofuscin pigment concentration in mice. J. Nutr. 1977;107(10):1792-1799.CrossRefGoogle Scholar
  6. 6.
    Fattoretti P, Bertoni-Freddari C, Casoli T, Di Stefano G, Solazzi M, Corvi E. Morphometry of age pigment (lipofuscin) and of ceroid pigment deposits associated with vitamin E deficiency. Arch. Gerontol. Geriatr. 2002;34(3):263-268.CrossRefGoogle Scholar
  7. 7.
    Hagiwara K, Naito K, Kurokawa Y, Ichikawa T. Kidney injury induced by lipid peroxide produced by vitamin E deficiency and GSH depletion in rats. J. Nutr. Sci. Vitaminol. (Tokyo). 1991;37(1):99-107.CrossRefGoogle Scholar
  8. 8.
    Höhn A, Grune T. Lipofuscin: formation, effects and role of macroautophagy. Redox Biol. 2013;1:140-144.CrossRefGoogle Scholar
  9. 9.
    Höhn A, Weber D, Jung T, Ott C, Hugo M, Kochlik B, Kehm R, König J, Grune T, Castro JP. Happily (n)ever after: Aging in the context of oxidative stress, proteostasis loss and cellular senescence. Redox Biol. 2017;11:482-501.CrossRefGoogle Scholar
  10. 10.
    Ivy GO, Kanai S, Ohta M, Smith G, Sato Y, Kobayashi M, Kitani K. Lipofuscin-like substances accumulate rapidly in brain, retina and internal organs with cysteine protease inhibition. Adv. Exp. Med. Biol. 1989;266:31-45.PubMedGoogle Scholar
  11. 11.
    Kikugawa K, Beppu M, Kato T, Yamaki S, Kasai H. Accumulation of autofluorescent yellow lipofuscin in rat tissues estimated by sodium dodecylsulfate extraction. Mech. Ageing Dev. 1994;74(1-2):135-148.CrossRefGoogle Scholar
  12. 12.
    Nandhini AT, Balakrishnan SD, Anuradha CV. Response of liver antioxidant system to taurine in rats fed high fructose diet. Indian J. Exp. Biol. 2002;40(9):1016-1019.PubMedGoogle Scholar
  13. 13.
    Reddy JK, Lalwani ND, Reddy MK, Qureshi SA. Excessive accumulation of autofluorescent lipofuscin in the liver during hepatocarcinogenesis by methyl clofenapate and other hypolipidemic peroxisome proliferators. Cancer Res. 1982;42(1):259-266.PubMedGoogle Scholar
  14. 14.
    Sato T, Tokoro Y, Tauchi H, Kohtani K, Mizuno T, Shimasaki H, Ueta N. Morphometrical and biochemical analysis on autofluorescent granules in various tissues and cells of the rats under several nutritional conditions. Mech. Ageing Dev. 1988;43(3):229-238.CrossRefGoogle Scholar
  15. 15.
    Wong SK, Chin KY, Suhaimi FH, Fairus A, Ima-Nirwana S. Animal models of metabolic syndrome:a review. Nutr. Metab. (Lond). 2016;13:65.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • S. A. Apryatin
    • 1
  • M. O. Semin
    • 1
  • I. V. Gmoshinskii
    • 1
    Email author
  • D. B. Nikityuk
    • 1
  1. 1.Federal Research Center of Nutrition and BiotechnologyMoscowRussia

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