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Characteristics of Artificial Immunogens Containing Peptide Mimotopes of HIV-1 Epitopes Recognized by Monoclonal Antibodies 2F5 and 2G12

The paper describes construction of TBI-based recombinant proteins TBI-2F5 and TBI-2G12 that contain peptide mimotopes of HIV-1 epitopes recognized by broadly neutralizing antibodies 2F5 and 2G12, respectively. The capacity of the immunogens to induce neutralizing antibodies was evaluated. The sera of BALB/c mice immunized with recombinant proteins TBI, TBI-2F5, and TBI-2G12 neutralized HIV-1 env-pseudoviruses. Moreover, pooled serum from mice immunized with TBI-2F5 and TBI-2G12 neutralized env-pseudoviruses of HIV-1 subtype B more effectively than individual sera.

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Correspondence to N. S. Shcherbakova.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 167, No. 2, pp. 213-217, February, 2019

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Shcherbakova, N.S., Chikaev, A.N., Rudometov, A.P. et al. Characteristics of Artificial Immunogens Containing Peptide Mimotopes of HIV-1 Epitopes Recognized by Monoclonal Antibodies 2F5 and 2G12. Bull Exp Biol Med 167, 259–262 (2019).

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Key Words

  • HIV-1
  • monoclonal antibodies 2F5 and 2G12
  • HIV-1 immunogen
  • phage display