Cellular Composition and Expression of Cell Proliferation and Apoptosis Markers in Bone Marrow Cells after Heterotransplantation
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The effects of heterotransplantation on bone marrow structures were studied in experimental male mice. Suspension of the bone marrow from feline femur (1 ml bone marrow diluted in isotonic saline, 1:2) was injected into the caudal vein. Heterotransplantation led to an increase in the level of serotonin involved in cell differentiation. In 2 days, the counts of all cells except erythroid cells decreased. Immunohistochemical analysis of Ki-67 marker within 40 min after transplantation showed a significant increase of cell proliferative activity in comparison with that in intact mice. At later terms, the expression of Ki-67 in bone marrow cells decreased, while the expression of apoptosis regulator P-53 increased, which was associated with the presence of numerous dead cells.
Key Wordsbone marrow heterotransplantation 5-HT cell proliferation apoptosis
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- 1.Vorob’eva OV. Neurotransmitters biogenic amines in the bone marrow structures at the allografting. Morfol. Ved. 2016;24(4):89-91. Russian.Google Scholar
- 2.Vorob’eva OV. Dynamics of the Morphofunctional State of Bone Marrow Cell-Differons as a Hematopoietic Organ. Zh. Anat. Gistopatol. 2017;6(2):26-29. Russian.Google Scholar
- 3.Karkishchenko NN, Gracheva SV. Guidelines for Laboratory Animals and Alternative Models in Biomedical Technologies. Moscow, 2010. Russian.Google Scholar
- 4.Lyubovtseva LA, Borisov AV. The effect of 5-HT on bone marrow cell proliferation. Morphology and Luminescent Histochemistry. Cheboksary, 1983. P. 124-126. Russian.Google Scholar
- 5.Lyubovtseva IA, Vorobeva OV, Lyubovtseva EV. Microanalysis of neurotransmitters in the introduction of foreign bone marrow. Morfol. Ved. 2015;(3):41-45. Russian.Google Scholar
- 6.Lyubovtseva EV, Lyubovtseva LA, Gur’yanova EA, Golubtsova NN, Moskovskii AV, Russov AV. The local regulation of organs by bioamine-containing cells. Morfologiya. 2009;136(4):91b. Russian.Google Scholar
- 8.Chesnokova NP, Morrison VV, Ponukalina EV, Zhevak TN, Afanas’eva GA, Polutova NV, Nevvazhai TA. Hematopoiesis and its regulation at various stages of differentiation of bone marrow hematopoietic cells. Saratov. Nauch.-Med. Zh. 2012;8(3):711-719. Russian.Google Scholar
- 9.Shur VY, Samotrueva MA, Mazhitova MV, Trizno NN, Fayziev RM, Petrenko LV, Shur YV. 5-HT: biological properties and prospects of clinical application. Fundament. Issled. 2014;(7-3):621-629. Russian.Google Scholar
- 10.Falck B, Hillarp NA, Thieme G, Torp A. Fluorescence of catechol amines and related compounds condensed with formaldehyde. J. Histochem. Cytochem. 1982;9(1-6):XI-XV.Google Scholar
- 11.Gerdes J. Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies. Semin. Cancer Biol. 1990;1(3):199-206.Google Scholar