Advertisement

Bulletin of Experimental Biology and Medicine

, Volume 166, Issue 5, pp 695–698 | Cite as

Cellular Composition and Expression of Cell Proliferation and Apoptosis Markers in Bone Marrow Cells after Heterotransplantation

  • O. V. Vorob’yovaEmail author
  • L. A. Lyubovtseva
Article
  • 7 Downloads

The effects of heterotransplantation on bone marrow structures were studied in experimental male mice. Suspension of the bone marrow from feline femur (1 ml bone marrow diluted in isotonic saline, 1:2) was injected into the caudal vein. Heterotransplantation led to an increase in the level of serotonin involved in cell differentiation. In 2 days, the counts of all cells except erythroid cells decreased. Immunohistochemical analysis of Ki-67 marker within 40 min after transplantation showed a significant increase of cell proliferative activity in comparison with that in intact mice. At later terms, the expression of Ki-67 in bone marrow cells decreased, while the expression of apoptosis regulator P-53 increased, which was associated with the presence of numerous dead cells.

Key Words

bone marrow heterotransplantation 5-HT cell proliferation apoptosis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Vorob’eva OV. Neurotransmitters biogenic amines in the bone marrow structures at the allografting. Morfol. Ved. 2016;24(4):89-91. Russian.Google Scholar
  2. 2.
    Vorob’eva OV. Dynamics of the Morphofunctional State of Bone Marrow Cell-Differons as a Hematopoietic Organ. Zh. Anat. Gistopatol. 2017;6(2):26-29. Russian.Google Scholar
  3. 3.
    Karkishchenko NN, Gracheva SV. Guidelines for Laboratory Animals and Alternative Models in Biomedical Technologies. Moscow, 2010. Russian.Google Scholar
  4. 4.
    Lyubovtseva LA, Borisov AV. The effect of 5-HT on bone marrow cell proliferation. Morphology and Luminescent Histochemistry. Cheboksary, 1983. P. 124-126. Russian.Google Scholar
  5. 5.
    Lyubovtseva IA, Vorobeva OV, Lyubovtseva EV. Microanalysis of neurotransmitters in the introduction of foreign bone marrow. Morfol. Ved. 2015;(3):41-45. Russian.Google Scholar
  6. 6.
    Lyubovtseva EV, Lyubovtseva LA, Gur’yanova EA, Golubtsova NN, Moskovskii AV, Russov AV. The local regulation of organs by bioamine-containing cells. Morfologiya. 2009;136(4):91b. Russian.Google Scholar
  7. 7.
    Nefedova VV, Inzhevatkin EV, Nefedov VP. Role of S2 receptors in the stimulatory effect of 5-HT on hemopoietic bone marrow stem cells. Bull. Exp. Biol. Med. 2002;133(5):419-420.CrossRefGoogle Scholar
  8. 8.
    Chesnokova NP, Morrison VV, Ponukalina EV, Zhevak TN, Afanas’eva GA, Polutova NV, Nevvazhai TA. Hematopoiesis and its regulation at various stages of differentiation of bone marrow hematopoietic cells. Saratov. Nauch.-Med. Zh. 2012;8(3):711-719. Russian.Google Scholar
  9. 9.
    Shur VY, Samotrueva MA, Mazhitova MV, Trizno NN, Fayziev RM, Petrenko LV, Shur YV. 5-HT: biological properties and prospects of clinical application. Fundament. Issled. 2014;(7-3):621-629. Russian.Google Scholar
  10. 10.
    Falck B, Hillarp NA, Thieme G, Torp A. Fluorescence of catechol amines and related compounds condensed with formaldehyde. J. Histochem. Cytochem. 1982;9(1-6):XI-XV.Google Scholar
  11. 11.
    Gerdes J. Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies. Semin. Cancer Biol. 1990;1(3):199-206.Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of General and Clinical Morphology and Forensic MedicineI. N. Ulyanov Chuvash State UniversityCheboksaryRussia

Personalised recommendations