Bulletin of Experimental Biology and Medicine

, Volume 166, Issue 5, pp 695–698 | Cite as

Cellular Composition and Expression of Cell Proliferation and Apoptosis Markers in Bone Marrow Cells after Heterotransplantation

  • O. V. Vorob’yovaEmail author
  • L. A. Lyubovtseva

The effects of heterotransplantation on bone marrow structures were studied in experimental male mice. Suspension of the bone marrow from feline femur (1 ml bone marrow diluted in isotonic saline, 1:2) was injected into the caudal vein. Heterotransplantation led to an increase in the level of serotonin involved in cell differentiation. In 2 days, the counts of all cells except erythroid cells decreased. Immunohistochemical analysis of Ki-67 marker within 40 min after transplantation showed a significant increase of cell proliferative activity in comparison with that in intact mice. At later terms, the expression of Ki-67 in bone marrow cells decreased, while the expression of apoptosis regulator P-53 increased, which was associated with the presence of numerous dead cells.

Key Words

bone marrow heterotransplantation 5-HT cell proliferation apoptosis 


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of General and Clinical Morphology and Forensic MedicineI. N. Ulyanov Chuvash State UniversityCheboksaryRussia

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