Bulletin of Experimental Biology and Medicine

, Volume 166, Issue 5, pp 676–679 | Cite as

Expression of Aβ42, τ-Protein, p16, p53 in Buccal Epithelium: Prospects for Use in the Diagnostics of Alzheimer’s Disease and Rate of Aging

  • V. A. Zuev
  • A. S. Dyatlova
  • N. S. Lin’kovaEmail author
  • T. V. Kvetnaya

The expression of Aβ42, and τ-protein, and p16 and p53 proteins was analyzed in the buccal epithelium of elderly and senile patients with Alzheimer’s disease. We revealed enhanced synthesis of Alzheimer’s disease markers Aβ42 (by 15-30 times) and τ-protein (by 5 times) in comparison with the corresponding values in people without neurodegenerative pathology of the same age groups. In addition, increased synthesis of proteins of cell aging and apoptosis p16 (by 6-10 times) and p53 (by 2-3 times) was observed in patients in comparison with age-matched persons without neuropathology. These data suggest that complex analysis of the expression of Aβ42, τ-protein, p16, and p53 in the buccal epithelium is a promising method for in vivo diagnosis of Alzheimer’s disease and assessment of the rate of aging during the development of this pathology.

Key Words

buccal epithelium Alzheimer’s disease Aβ42 protein τ-protein aging 


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  1. 1.
    Ankarcrona M, Winblad B, Monteiro C, Fearns C, Powers ET, Johansson J, Westermark GT, Presto J, Ericzon BG, Kelly JW. Current and future treatment of amyloid diseases. J. Intern. Med. 2016;280(2):177-202.CrossRefGoogle Scholar
  2. 2.
    Armstrong RA. A critical analysis of the ‘amyloid cascade hypothesis’. Folia Neuropathol. 2014;52(3):211-225.CrossRefGoogle Scholar
  3. 3.
    Forero DA, González-Giraldo Y, López-Quintero C, Castro-Vega LJ, Barreto GE, Perry G. Meta-analysis of telomere length in Alzheimer’s disease. J. Gerontol. A Biol. Sci. Med. Sci. 2016;71(8):1069-1073.CrossRefGoogle Scholar
  4. 4.
    François M, Fenech MF, Thomas P, Hor M, Rembach A, Martins RN, Rainey-Smith SR, Masters CL, Ames D, Rowe CC, Macaulay SL, Hill AF, Leifert WR, The Australian Imaging Biomarkers and Lifestyle Study Research Group. High content, multi-parameter analyses in buccal cells to identify Alzheimer’s disease. Curr. Alzheimer Res. 2016;13(7):787-799.CrossRefGoogle Scholar
  5. 5.
    François M, Leifert W, Martins R, Thomas P, Fenech M. Biomarkers of Alzheimer’s disease risk in peripheral tissues; focus on buccal cells. Curr. Alzheimer Res. 2014;11(6):519-531.CrossRefGoogle Scholar
  6. 6.
    Garcia A, Mathur S, Kalaw MC, McAvoy E, Anderson J, Luedke A, Itorralba J, Mai S. Quantitative 3D telomeric imaging of buccal cells reveals Alzheimer’s disease-specific signatures. J. Alzheimers Dis. 2017;58(1):139-145.CrossRefGoogle Scholar
  7. 7.
    Hartmann S, Möbius HJ. Tolerability of memantine in combination with cholinesterase inhibitors in dementia therapy. Int. Clin. Psychopharmacol. 2003;18(2):81-85.CrossRefGoogle Scholar
  8. 8.
    Hattori H, Matsumoto M, Iwai K, Tsuchiya H, Miyauchi E, Takasaki M, Kamino K, Munehira J, Kimura Y, Kawanishi K, Hoshino T, Murai H, Ogata H, Maruyama H, Yoshida H. The tau protein of oral epithelium increases in Alzheimer’s disease. J. Gerontol. A Biol. Sci. Med. Sci. 2002;57(1):M64-M70.CrossRefGoogle Scholar
  9. 9.
    Khan TK, Sen A, Hongpaisan J. PKCε deficits in Alzheimer’s disease brains and skin fibroblasts. J. Alzheimers Dis. 2015;43(2):491-509.CrossRefGoogle Scholar
  10. 10.
    Lovat LB, Persey MR, Madhoo S, Pepys MB, Hawkins PN. The liver in systemic amyloidosis: insights from 123I serum amyloid P component scintigraphy in 484 patients. Gut. 1998;42(5):727-734.CrossRefGoogle Scholar
  11. 11.
    Micheli F, Palermo R, Talora C, Ferretti E, Vacca A, Napolitano M. Regulation of proapoptotic proteins Bak1 and p53 by miR-125b in an experimental model of Alzheimer’s disease: Protective role of 17β-estradiol. Neurosci. Lett. 2016;629:234-240.CrossRefGoogle Scholar
  12. 12.
    O’Brien RJ, Wong PC. Amyloid precursor protein processing and Alzheimer’s disease. Annu. Rev. Neurosci. 2011;34:185-204.CrossRefGoogle Scholar
  13. 13.
    Sajan FD, Martiniuk F, Marcus DL, Frey WH, Hite R, Bordayo EZ, Freedman ML. Apoptotic gene expression in Alzheimer’s disease hippocampal tissue. Am. J. Alzheimers Dis. Other Demen. 2007;22(4):319-328.CrossRefGoogle Scholar
  14. 14.
    Schott K, Wormstall H, Dietrich M, Klein R, Batra A. Autoantibody reactivity in serum of patients with Alzheimer’s disease and other age-related dementias. Psychiatry. 1996;59(3):251-254.Google Scholar
  15. 15.
    Thomas P, Hecker J, Faunt J, Fenech M. Buccal micronucleus cytome biomarkers may be associated with Alzheimer’s disease. Mutagenesis. 2007;22(6):371-379.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • V. A. Zuev
    • 1
  • A. S. Dyatlova
    • 1
    • 2
  • N. S. Lin’kova
    • 1
    • 2
    Email author
  • T. V. Kvetnaya
    • 1
  1. 1.St. Petersburg Research Center Institute of Bioregulation and GerontologySt. PetersburgRussia
  2. 2.Peter the Great St. Petersburg Polytechnic UniversitySt. PetersburgRussia

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