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Bulletin of Experimental Biology and Medicine

, Volume 166, Issue 5, pp 637–640 | Cite as

Death Mechanisms of Pulmonary Alveolocytes in Mice Infected with Influenza Viruses A/H1N1/California/04/2009 and A/H5N1/Goose/Krasnoozerskoye/627/05

  • L. A. CherdantsevaEmail author
  • A. V. Kovner
  • T. V. Sharkova
  • V. A. Shkurupy
  • A. M. Shestopalov
  • O. V. Potapova
Article

In CBA mice infected with influenza viruses A/H1N1/California/04/2009 and A/H5N1/Goose/Krasnoozerskoye/627/05 in a dose of 10 MLD50, the mechanisms of death of pulmonary alveolocytes over 10 postinfection days were studied by light microscopy, immunohistochemistry, and morphometry. In mice infected with A/H1N1, alveolocytes died predominantly via necrosis, while apoptosis mostly employed the mitochondrial pathway. In mice infected with A/H5N1, apoptosis was the dominant mechanism of alveolocyte death proceeded via membrane receptor signaling followed by switching to FAS-mediated pathway via activation of FADD, the apoptotic signal transduction protein.

Key Words

alveolocytes influenza A viruses apoptosis necrosis caspases 9, 3 

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References

  1. 1.
    Domeniconi M, Hempstead BL, Chao MV. Pro-NGF secreted by astrocytes promotes motor neuron cell death. Mol. Cell. Neurosci. 2007;34(2):271-279.CrossRefGoogle Scholar
  2. 2.
    Kacergius T, Ambrozaitis A, Deng Y, Gravenstein S. Neuraminidase inhibitors reduce nitric oxide production in influenza virus-infected and gamma interferon-activated RAW 264.7 macrophages. Pharmacol. Rep. 2006;58(6):924-930.Google Scholar
  3. 3.
    Kovner AV, Anikina AG, Potapova OV, Sharkova TV, Cherdanceva LA, Shkurupy VA, Shestopalov AM. Structural and functional changes in pulmonary macrophages and lungs of mice infected with influenza virus A/H5N1 A/goose/Krasnoozerskoye/627/05. Bull. Exp. Biol. Med. 2012;153(2):229-232.CrossRefGoogle Scholar
  4. 4.
    Kovner AV, Potapova OV, Shkurupy VA. Morphofunctional changes in the lung vascular endotheliocytes in mice with influenza A/H5N1 A/Goose/Krasnoozerskoye/627/05. Bull. Exp. Biol. Med. 2013;154(4):476-479.CrossRefGoogle Scholar
  5. 5.
    Kovner AV, Potapova OV, Shkurupy VA, Shestopalov AM. Morphofunctional characteristics of pulmonary surfactant system and its effect on immune cells in influenza A (H1N1) pathogenesis. Open J. Pathol. 2016;6(1):1-7.CrossRefGoogle Scholar
  6. 6.
    Kuiken T, Rimmelzwaan GF, Van Amerongen G, Osterhaus AD. Pathology of human influenza A (H5N1) virus infection in cynomolgus macaques (Macaca fascicularis). Vet. Pathol. 2003;40(3):304-310.CrossRefGoogle Scholar
  7. 7.
    Lasso Pirot A, Fritz KI, Ashraf QM, Mishra OP, Delivoria-Papadopoulos M. Effects of severe hypocapnia on expression of Bax and Bcl-2 proteins, DNA fragmentation, and membrane peroxidation products in cerebral cortical mitochondria of newborn piglets. Neuropathology. 2007;91(1):20-27.Google Scholar
  8. 8.
    Sreta D, Kedkovid R, Tuamsang S, Kitikoon P, Thanawongnuwech R. Pathogenesis of swine influenza virus (Thai isolates) in weanling pigs: an experimental trial. Virol. J. 2009;6:34. doi:  https://doi.org/10.1186/1743-422X-6-34.CrossRefGoogle Scholar
  9. 9.
    Potapova OV, Kovner AV, Anikina AG, Cherdantseva LA, Sharkova TV, Shkurupy VA, Vasil’eva EV, Shestopalov AM. Studies of influenza A/H1N1 A/Tomsk/13/2010 virus topology during development of infectious process in mammals. Bull. Exp. Biol. Med. 2016;160(5. Р. 683-686.Google Scholar
  10. 10.
    Xu T, Qiao J, Zhao L, Wang G, He G, Li K, Tian Y, Gao M, Wang J, Wang H, Dong C. Acute respiratory distress syndrome induced by avian influenza A (H5N1) virus in mice. Am. J. Respir. Crit. Care Med. 2006;174(9):1011-1017.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • L. A. Cherdantseva
    • 1
    Email author
  • A. V. Kovner
    • 1
  • T. V. Sharkova
    • 2
  • V. A. Shkurupy
    • 1
    • 3
  • A. M. Shestopalov
    • 1
  • O. V. Potapova
    • 2
  1. 1.Research Institute of Experimental and Clinical Medicine, Federal Research Center of Fundamental and Translational MedicineNovosibirskRussia
  2. 2.Optimum Medical Laboratory, Research-and-Educational CenterSochiRussia
  3. 3.Novosibirsk State Medical UniversityNovosibirskRussia

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