Bulletin of Experimental Biology and Medicine

, Volume 166, Issue 5, pp 626–630 | Cite as

Comparison of Profiles of Extracellular MicroRNA Secreted by Caco-2 Cells from the Apical Side of the Membrane under Static and Microcirculation Conditions

  • S. V. NikulinEmail author
  • T. N. Gerasimenko
  • S. A. Shilin
  • I. N. Gazizov
  • O. V. Kindeeva
  • D. A. Sakharov

Extracellular microRNA are one of the indicators of the functional state of cells. Culturing of Caco-2 cells under the conditions of microcirculation in a Homunculus microfluidic device allows better simulating natural environment of the body in comparison with static culturing. Impedance spectroscopy (BioClinicum Research Center) was used for non-invasive estimation of the monolayer quality and changes in the cell apical membrane due to the formation of microvilli. Under static conditions, Caco-2 cells release more microRNA from the apical membrane than under microcirculation conditions, while secretion of miR-320a, miR-24-3p, and miR-221-3p microRNA under static conditions can indicate stress of the cells and activation of inflammatory response. Under microcirculation conditions, the expression of laminin-α1 (LAMA1) was lower than under static conditions, which indicates deeper differentiation of cells.

Key Words

impedance spectroscopy TEER Caco-2 microcirculation microfluidicic device 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • S. V. Nikulin
    • 1
    Email author
  • T. N. Gerasimenko
    • 1
  • S. A. Shilin
    • 1
  • I. N. Gazizov
    • 1
    • 2
  • O. V. Kindeeva
    • 1
    • 2
  • D. A. Sakharov
    • 1
  1. 1.BioClinicum Research CenterMoscowRussia
  2. 2.Far-Easter Federal UniversityVladivostokRussia

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