Bulletin of Experimental Biology and Medicine

, Volume 166, Issue 5, pp 598–601 | Cite as

The Role of NO Synthase in the Cardioprotective Effect of Substances of Humic Origin on the Model of Ischemia and Reperfusion of Isolated Rat Heart

  • T. V. LasukovaEmail author
  • M. V. Zykova
  • M. V. Belousov
  • A. S. Gorbunov
  • L. A. Logvinova
  • A. M. Dygai

The cardioprotective and inotropic effects of standardized active natural substance based on high-molecular-weight compounds of humic origin were studied on the model of global ischemia (40 min) and reperfusion of isolated perfused rat heart. Preventive administration of the test substance (0.1 mg/ml) before ischemia/reperfusion modeling reduced reperfusion contracture and necrotic death of cardiomyocytes and promoted recovery of myocardial contractility. Blockade of NO synthase with L-NAME (100 μM) abolished the above effects of the test substance. It was hypothesized that NO synthase plays an important role in the development of the cardioprotective and inotropic effects of the test natural substance.

Key Words

active substance heart ischemia reperfusion NO synthase 


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • T. V. Lasukova
    • 1
    • 2
    Email author
  • M. V. Zykova
    • 3
  • M. V. Belousov
    • 3
  • A. S. Gorbunov
    • 2
  • L. A. Logvinova
    • 3
  • A. M. Dygai
    • 4
  1. 1.Tomsk State Pedagogical UniversityTomskRussia
  2. 2.Research Institute of Cardiology, Tomsk National Research Medical CenterRussian Academy of SciencesTomskRussia
  3. 3.Siberian State Medical University, Ministry of Health of the Russian FederationTomskRussia
  4. 4.E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical CenterRussian Academy of SciencesTomskRussia

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