Effect of Activated Immunocompetent Cells on the Number of Multipotent Stromal Cells in Bone Marrow Transplants of CBA and CBA/N Mice in a Short Time after Polyvinylpyrrolidone Administration to Animals
- 3 Downloads
One hour after polyvinylpyrrolidone administration, the content of multipotent stromal cells in the spleen of CBA and CBA/N mice increased almost equally (by 2.5 and 2.9 times, respectively), but in 24 h, the effectiveness of multipotent stromal cell cloning in the spleen of CBA/N mice decreased almost to the control level, whereas in CBA mice, the number of multipotent stromal cells continued to increase. Serum concentration of IL-5, TNFα, and IL-2 in both lines was elevated in 1 h after polyvinylpyrrolidone administration, which is likely to reflect activation of the innate immunity. One day after polyvinylpyrrolidone administration, the number of multipotent stromal cells in bone marrow transplants in the CBA/N→CBA/N and CBA→CBA/N groups remained practically unchanged, while in groups CBA→CBA and CBA/N→CBA it was equally increased (by 3.6 and 3.4 times, respectively). Thus, the number of multipotent stromal cells in bone marrow transplants after 1 day was increased only in groups where recipients (CBA mice) were capable of responding to polyvinylpyrrolidone administration, i.e. the number of stromal cells by this term, was apparently determined by the presence of activated immunocompetent cells. These findings also indicate that activation of the stromal tissue dur ing immune response can have a two-phasic pattern: the first phase (1 h after antigen adminis tration) can be determined by activation of innate immunity receptors (in multipotent stromal cells or other cells) observed in CBA and CBA/N mice, and the second phase occurs during further development of the immune response (that was observed in CBA mice, but not in CBA/N mice due to absence of CD+B-1a lymphocytes). The findings attest to close interactions between the stromal tissue and the immune system.
Key Wordsstromal cells MSC immune response
Unable to display preview. Download preview PDF.
- 1.Gorskaya YF, Danilova TA, Karyagina AS, Lunin VG, Grabko VI, Bartov MS, Gromov AV, Grunina TM, Soboleva LA, Shapoval IM, Nesterenko VG. Effects of combined treatment with complex S. typhimurium antigens and factors stimulating osteogenesis (curettage, BMP-2) on multipotent bone marrow stromal cells and serum concentration of cytokines in CBA mice. Bull. Exp. Biol. Med. 2015;158(4):456-470.CrossRefGoogle Scholar
- 2.Gorskaya UF, Danilova TA, Mesentzeva MV, Shapoval IM, Narovlyansky AN, Nesterenko VG. Number of stromal precursors in mouse bone marrow and expression of cytokine genes in primary cultures of mouse bone marrow cells during various periods after immunization with S. typhimurium antigens. Bull. Exp. Biol. Med. 2010;149(4):425-427.CrossRefGoogle Scholar
- 3.Gorskaya UF, Danilova TA, Mezentzeva MV, Shapoval MM, Nesterenko VG. Effect of immunization with polyvinylpyrrolidone on the counts of stromal precursor cells in bone marrow and spleen of CBA and CBA/N mice and cytokine gene expression in primary cultures of these cells. Bull. Exp. Biol. Med. 2012;153(1):64-67.CrossRefGoogle Scholar
- 4.Gorskaya YF, Tukhvatulin AI, Nesterenko VG. NLR2 and TLR3, TLR4, TLR5 Ligands, Injected In Vivo, Improve after 1 h the Efficiency of Cloning and Proliferative Activity of Bone Marrow Multipotent Stromal Cells and Reduce the Content of Osteogenic Multipotent Stromal Cells in CBA Mice. Bull. Exp. Biol. Med. 2017;163(3):356-360.CrossRefGoogle Scholar
- 5.Friedenstein AJ, Luria EA. Cellular Basis of Hematopoietic Microenvironment. Moscow, 1980. Russian.Google Scholar
- 7.Prior L, Pierson S, Woodland RT, Riggs J. Rapid restoration of B-cell function in XID mice by intravenous transfer of peritoneal cavity B cells. J. Immunology. 1994;83(2):180-183.Google Scholar