Oxidative status was assessed in different areas of the cerebral cortex of male Wistar rats under normal condition and during permanent 24-h focal ischemia. In intact animals, the level of lipid hydroperoxides in the frontal lobes of both hemispheres was by 36% higher than in other cortical areas, while total antioxidant activity was by 25% higher than in other areas. During ischemia, changes in oxidative status were localized only in the ischemic focus and penumbra zone and did not involve other cortical areas. We demonstrated for the first time a neuroprotective effect of therapeutic administration of carnosine in low doses (50 mg/kg) on parameters of the oxidative status under conditions of focal ischemia comparable to its effect of high doses (500 mg/kg) as well as its local effect in the penumbra zone. A dose-dependent effect of carnosine on antioxidant activity in the penumbra zone during ischemia was also demonstrated. These findings confirm effectiveness of not only preventive carnosine administration, but also its application in the postischemic period of the stroke.
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References
Boldyrev AA. Carnosine: New concept for the function of an old molecule. Biochemistry (Moscow). 2012;77(4):313-326.
Gavrilova SA, Samoilenkova NS, Pirogov YuA, Khudoerkov RM, Koshelev VB. Neuroprotective effect of hypoxic preconditioning in focal brain ischemia. Patogenez. 2008;6(3):13-17. Russian.
Gusev EI, Skvortsova VI. Brain ischemia. Moscow, 2001. Russian.
Gus’kova TA, Fizenko VP. Guidelines for evaluation of the safety of drugs in the clinic on the basis of the results of preclinical toxicological experiments. Manual on Experimental (Preclinical) Study of New Pharmacological Substances, Khabriev RU, ed. Moscow, 2005. P. 105-106. Russian.
Fedorova TN, Boldyrev AA, Gannushkina IV. Lipid peroxidation in experimental ischemia of the brain. Biochemistry (Mosc). 1999;64(1):75-79.
Fedorova TN, Gavrilova SA, Morozova MP, Devyatov AA, Berezhnoy DS, Stvolinsky SL. The neuroprotective effect of the carnosine in a focal cerebral ischemia. Vopr. Biol. Med. Farm. Khimii. 2017;20(4):25-32. Russian.
Beghoul A, Kebieche M, Gasmi S, Chouit Z, Amiour C, Lahouel A, Lakroun Z, Rouabhi R, Fetoui H, Soulimani R. Impairment of mitochondrial integrity and redox status in brain regions during a low-dose long-term exposition of rats to pyrethrinoïds: the preventive effect of quercetin. Environ. Sci. Pollut. Res. Int. 2017;24(24):19,714-19,722.
Bonova P, Burda J, Danielisova V, Nemethova M, Gottlieb M. Development of a pattern in biochemical parameters in the core and penumbra during infarct evolution after transient MCAO in rats. Neurochem. Int. 2013;62(1):8-14.
Davis CK, Laud PJ, Bahor Z, Rajanikant GK, Majid A. Systematic review and stratified meta-analysis of the efficacy of carnosine in animal models of ischemic stroke. J. Cereb. Blood Flow Metab. 2016;36(10):1686-1694.
Doyle KP, Simon RP, Stenzel-Poore MP. Mechanisms of ischemic brain damage. Neuropharmacology. 2008;55(3):310-318.
Lausada N, Arnal N, Astiz M, Marín MC, Lofeudo JM, Stringa P, Tacconi de Alaniz MJ, Tacconi de Gómez Dumm N, Hurtado de Catalfo G, Cristalli de Piñero N, Pallanza de Stringa MC, Illara de Bozzolo EM, Bozzarello EG, Cristalli DO, Marra CA. Dietary fats significantly influence the survival of penumbral neurons in a rat model of chronic ischemic by modifying lipid mediators, inflammatory biomarkers, NOS production, and redox-dependent apoptotic signals. Nutrition. 2015;31(11-12):1430-1442.
Majid A. Neuroprotection in stroke: past, present, and future. ISRN Neurol. 2014;2014. ID 515716. doi: https://doi.org/10.1155/2014/515716.
McCann SK, Dusting GJ, Roulston CL. Early increase of Nox4 NADPH oxidase and superoxide generation following endothelin-1-induced stroke in conscious rats. J. Neurosci. Res. 2008;86(11):2524-2534.
Nayak P, Sharma SB, Chowdary NV. Aluminum and ethanol induce alterations in superoxide and peroxide handling capacity (SPHC) in frontal and temporal cortex. Indian J. Biochem. Biophys. 2013;50(5):402-410.
Tomizawa S, Imai H, Tsukada S, Simizu T, Honda F, Nakamura M, Nagano T, Urano Y, Matsuoka Y, Fukasaku N, Saito N. The detection and quantification of highly reactive oxygen species using the novel HPF fluorescence probe in a rat model of focal cerebral ischemia. Neurosci. Res. 2005;53(3):304-313.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 165, No. 6, pp. 703-708, June, 2018
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Fedorova, T.N., Devyatov, A.A., Berezhnoi, D.S. et al. Oxidative Status in Different Areas of the Cerebral Cortex of Wistar Rats during Focal Ischemia and Its Modulation with Carnosine. Bull Exp Biol Med 165, 746–750 (2018). https://doi.org/10.1007/s10517-018-4256-x
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DOI: https://doi.org/10.1007/s10517-018-4256-x