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Bulletin of Experimental Biology and Medicine

, Volume 165, Issue 3, pp 382–385 | Cite as

Specific Features of Transcription Activity of Cancer-Testis Antigens in Patients with Metastatic and Non-Metastatic Breast Cancer

  • D. I. Vodolazhsky
  • D. S. Kutilin
  • Kh. A. Mogushkova
  • O. I. Kit
ONCOLOGY

Cancer-testis antigens, effective markers of tissue malignant transformation, are characterized by heterogonous transcription depending on the pathological features of breast cancer. We performed screening of transcription profile of cancer-testis antigens specific for breast tumor tissues in female patients with and without regional metastasis. The relative expression of 16 genes (MAGEA1, MAGEA2, MAGEA3, MAGEA4, MAGEB1, MAGEB2, GAGE1, GAGE3, GAGE4, MAGEC1, BAGE, XAGE3, NY-ESO1, SSX2, SYCP1, and PRAME1) was analyzed by RT-qPCR method in biopsy specimens of the mammary gland tissues obtained during surgery from 25 patients. Differential transcription activity of cancer-testis antigens genes was observed in patients with metastatic (enhanced expression of MAGEA2, MAGEB1, and XAGE3 genes) and non-metastatic (enhanced expression of GAGE3 and PRAME1 genes) breast cancer.

Key Words

gene expression real-time PCR cancer-testis antigens breast cancer metastases 

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • D. I. Vodolazhsky
    • 1
  • D. S. Kutilin
    • 1
  • Kh. A. Mogushkova
    • 1
  • O. I. Kit
    • 1
  1. 1.Rostov Research Institute of OncologyMinistry of Health of the Russian FederationRostov-on-DonRussia

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