Bulletin of Experimental Biology and Medicine

, Volume 165, Issue 3, pp 360–363 | Cite as

Dihydroquercetin and Fucoidin Inhibit the Increase of Angiotensin-Converting Enzyme Activity in the Rat Aorta after Irradiation

  • A. F. Korystova
  • L. N. Kublik
  • Yu. A. Kim
  • M. Kh. Levitman
  • V. V. Shaposhnikova
  • Yu. N. KorystovEmail author

The time course of angiotensin-converting enzyme activity in the rat aorta after fractionated exposure to ionizing radiation and the effects of dihydroquercetin and fucoidin on this parameter were studied. Male Wistar rats were exposed to single or repeated (fractionated) X-ray radiation in a dose of 2.5 Gy at 200 kV. Activity of angiotensin-converting enzyme in aorta segments was evaluated 2 h after the last exposure by hydrolysis of hippuryl-histidineleucin substrate. Enzyme activity in the rat aorta was higher than normally after all the studied doses of fractionated exposure (2.5 Gy per fraction) with the maximum effect after the total dose of 7.5 Gy (3 fractions). Fucoidin, a blocker of endothelium receptors realizing the leukocyte adhesion to the endothelium, and flavonoid dihydroquercetin inhibiting expression of adhesion molecules in the endothelium abolished the increase in activity of angiotensinconverting enzyme in the rat aorta after single exposure; moreover, dihydroquercetin reduced significantly the effect of fractionated exposure. These data indicate that leukocyte adhesion to the endothelium is an important factor contributing to the increase of angiotensin-converting enzyme activity in the aorta.

Key Words

aorta angiotensin-converting enzyme ionizing radiation 


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  1. 1.
    Arutyunyan TV, Korystova AF, Kublik LN, Levitman MKh, Shaposhnikova VV, Korystov YN. Taxifolin and Fucoidin Abolish the Irradiation-Induced Increase in the Production of Reactive Oxygen Species in Rat Aorta. Bull. Exp. Biol. Med. 2016;160(5):635-638.CrossRefPubMedGoogle Scholar
  2. 2.
    Korystova AF, Kublik LN, Levitman MKh, Samokhvalova TV, Shaposhnikova VV, Korystov YuN. Ionizing radiation enhances activity of angiotensin-converting enzyme in rat aorta. Bull. Exp. Biol. Med. 2018;165(2):178-182.CrossRefGoogle Scholar
  3. 3.
    Ackermann A, Fernández-Alfonso MS, Sánchez de Rojas R, Ortega T, Paul M, González C. Modulation of angiotensinconverting enzyme by nitric oxide. Br. J. Pharmacol. 1998; 124(2):291-298.CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Dzau VJ. Theodore Cooper Lecture: Tissue angiotensin and pathobiology of vascular disease: a unifying hypothesis. Hypertension 2001;37(4):1047-1052.CrossRefPubMedGoogle Scholar
  5. 5.
    Fleming I. Signaling by the angiotensin-converting enzyme. Circ. Res. 2006;98(7):887-896.CrossRefPubMedGoogle Scholar
  6. 6.
    Gerritsen ME, Carley WW, Ranges GE, Shen CP, Phan SA, Ligon GF, Perry CA. Flavonoids inhibit cytokine-induced endothelial cell adhesion protein gene expression. Am. J. Pathol. l995;147(2):278-292.Google Scholar
  7. 7.
    Hallahan D, Kuchibhotla J, Wyble C. Cell adhesion molecules mediate radiation-induced leukocyte adhesion to the vascular endothelium. Cancer Res. 1996;56(22):5150-5155.PubMedGoogle Scholar
  8. 8.
    Hiroshiba N, Ogura Y, Sasai K, Nishiwaki H, Miyamoto K, Hamada M, Tsujikawa A, Honda Y. Radiation-induced leukocyte entrapment in the rat retinal microcirculation. Invest. Ophthalmol. Vis. Sci. 1999;40(6):1217-1222.PubMedGoogle Scholar
  9. 9.
    Kanwar S, Bullard DC, Hickey MJ, Smith CW, Beaudet AL, Wolitzky BA, Kubes P. The association between alpha4-integrin, P-selectin, and E-selectin in an allergic model of inflammation. J. Exp. Med. 1997;185(6):1077-1087.CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Kojda G, Harrison D. Interactions between NO and reactive oxygen species: pathophysiological importance in atherosclerosis, hypertension, diabetes and heart failure. Cardiovasc. Res. 1999;43(3):562-571.CrossRefPubMedGoogle Scholar
  11. 11.
    Linz W, Wohlfart P, Schölkens BA, Malinski T, Wiemer G. Interactions among ACE, kinins and NO. Cardiovasc. Res. 1999;43(3):549-561.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • A. F. Korystova
    • 1
  • L. N. Kublik
    • 1
  • Yu. A. Kim
    • 2
  • M. Kh. Levitman
    • 1
  • V. V. Shaposhnikova
    • 1
  • Yu. N. Korystov
    • 1
    Email author
  1. 1.Institute of Theoretical and Experimental BiophysicsRussian Academy of SciencesPushchinoRussia
  2. 2.Institute of Cell BiophysicsRussian Academy of SciencesPushchinoRussia

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