Advertisement

Effect of Calcium on Slow Force Responses in Isolated Right Ventricle Preparations of Healthy and Hypertrophied Myocardium in Male and Female Rats

  • Yu. L. Protsenko
  • D. A. Kuznetsov
  • R. V. Lisin
  • O. N. Lukin
  • A. A. Balakin
Article
  • 1 Downloads

Effect of different Ca2+ concentrations in the bathing solution [Ca2+]o on the parameters of single isometric contraction and slow force response to stretching was studied in isolated preparations of healthy and hypertrophied myocardium of male and female Wistar rats. In all groups of experimental animals, the increase in calcium concentration was followed by a decrease in the myocardium slow response intensity. We revealed a complementary relationship between the current and medium-term systems of myocardial contractility regulation by the length of the myocardium aimed at the maintenance of the constant level during adaptation to the load. Slow responses of the hypertrophied rat heart myocardium were suppressed in comparison with those in the healthy myocardium and their intensity did not depend on animal sex.

Key Words

rat myocardium right ventricular hypertrophy monocrotaline slow force response changes in muscle length 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Protsenko YL, Balakin AA, Kuznetsov DA, Kursanov AG, Lisin RV, Mukhlynina EA, Lookin ON. Contractility of Right Ventricular Myocardium in Male and Female Rats during Physiological and Pathological Hypertrophy. Bull. Exp. Biol. Med. 2017;162(3):303-305.CrossRefPubMedGoogle Scholar
  2. 2.
    Balakin A, Lisin R, Smoluk A, Protsenko Yu. Alternative approach for studying contractility of the isolated muscle preparations in real-time mode. J. Biomed. Sci. Engineer. 2015;8:643-652.Google Scholar
  3. 3.
    Bassani RA, Gilioli R, Oliveira ES, Hoehr NF. Blood calcium levels in immature rats: influence of extracellular calcium concentration on myocardial calcium handling. Exp. Anim. 2012;61(4):399-405.CrossRefPubMedGoogle Scholar
  4. 4.
    Brunner F. Cardiac endothelin and big endothelin in rightheart hypertrophy due to monocrotaline-induced pulmonary hypertension in rat. Cardiovasc. Res. 1999;44(1):197-206.CrossRefPubMedGoogle Scholar
  5. 5.
    Cingolani HE, Pérez NG, Cingolani OH, Ennis IL. The Anrep effect: 100 years later. Am. J. Physiol. Heart Circ. Physiol. 2013;304(2):H175-H182.CrossRefPubMedGoogle Scholar
  6. 6.
    Escobar AL, Ribeiro-Costa R, Villalba-Galea C, Zoghbi ME, Pérez CG, Mejía-Alvarez R. Developmental changes of intracellular Ca 2+ transients in beating rat hearts. Am. J. Physiol. Heart Circ. Physiol. 2004;286(3):H971-H978.CrossRefPubMedGoogle Scholar
  7. 7.
    Shen X, Cannell MB, Ward ML. Effect of SR load and pH regulatory mechanisms on stretch-dependent Ca2+ entry during the slow force response. J. Mol. Cell. Cardiol. 2013;63:37-46.CrossRefPubMedGoogle Scholar
  8. 8.
    Tucci PJ, Faber CN, dos Santos L, Antonio EL. Slow inotropic response of intact left ventricle to sudden dilation critically depends on a myocardial dialysable factor. Clin. Exp. Pharmacol. Physiol. 2007;34(5-6):515-516.CrossRefPubMedGoogle Scholar
  9. 9.
    von Lewinski D, Kockskämper J, Zhu D, Post H, Elgner A, Pieske B. Reduced stretch-induced force response in failing human myocardium caused by impaired Na(+)-contraction coupling. Circ. Heart Fail. 2009;2(1):47-55.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Yu. L. Protsenko
    • 1
  • D. A. Kuznetsov
    • 1
  • R. V. Lisin
    • 1
  • O. N. Lukin
    • 1
  • A. A. Balakin
    • 1
  1. 1.Institute of Immunology and PhysiologyUral Division of the Russian Academy of SciencesEkaterinburgRussia

Personalised recommendations