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Biodistribution of Alpha-Fetoprotein-Containing Noncovalent Complex Aimpila with Antitumor Activity

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Biodistribution of [125I]Aimpila (20 mg/kg) in the tumor and normal tissues, including the mammary gland tissue, after single oral dose was studied in BALB/c nude mice with T47D/ReCAF+++ human breast tumor sensitive to this drug and in closely related BALB/c nude+mice without tumors. The maximum concentration of [125I]Aimpila was in fact the same in the tumor and in the mammary gland, while the time course of its accumulation/elimination differed. The time of the maximum accumulation of the drug in the tumor was shorter and its persistence longer than in normal tissue. After 24 h, label concentration in the tumor was 4.5 times higher (p=0.002). Differences in the time course of label accumulation in the tumor were detected. The maximum ratio of tumor/blood concentrations of the preparation was recorded in 1 h after administration. [125I]Aimpila and [125I]alpha-fetoprotein accumulated in the tumor in comparable concentrations and were eliminated simultaneously at the same rate. The results of comparative analysis of accumulation of the labeled compounds in Aimpila-sensitive T47D/RECAF+++ tumor from 0.5 to 9.0 h after drug administration could be interpreted as a result of possible receptor-mediated binding of the complex with the tumor at the expense of the alpha-fetoprotein transporting part. Differences in the parameters of [125I]Aimpila biodistribution in the tumor and normal mammary tissue indirectly attested to selective antiproliferative activity of the complex.

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References

  1. 1.

    Andronova NN, Tsurka SA, Cherkasova ZhR, Smirnova GB, Borisova YuA, Treshchalina EM. Bioavailability of oral α-fetoprotein-containing non-covalent complex Aimpila in the rat model of the isolated segment of the small intestine (inverted pouch). Ross. Bioter. Zh. 2016;15(1):6-7. Russian.

  2. 2.

    Andronova NB, Tcherkassova JR, Tsurkan SA, Smirnova GB, Treshalina HM. Evaluation of the internalization of AFP-containing noncovalent complexes AIMPILA in the rat model of the isolated segment of rat small intestine. Ross. Onkol. Zh. 2016;21(6):308-311. Russian.

  3. 3.

    Grigor’eva EYu, Tsurkan SA, Smirnova GB, Borisova YuA, Treshchalina EM. Peculiarities of pharmacokinetics of AFPcontaining complex Aimpila after oral administration. Ross. Bioter. Zh. 2016;15(1):26. Russian.

  4. 4.

    Medical Laboratory Technologies: a Guide to Clinical Laboratory Diagnostics. Karpishchenko AI, ed. Moscow, 2013. Vol. 2. Russian.

  5. 5.

    Smirnova GB, Tsurkan SA, Borisov YuA, Treshchalina EM. Efficacy of Aimpila preparation on subcutaneous xenografts of human tumors. Ross. Bioter. Zh. 2016;15(1):102. Russian.

  6. 6.

    Treshchalina EM. Immunodeficient mice bred at the N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences. Moscow, 2010. Russian.

  7. 7.

    Treshchalina EM. Collection of Strains of Human Tumors. Davydov MI, ed. Moscow, 2009. P. 59-64. Russian.

  8. 8.

    Cherkasova ZhR, Tsurkan SA, Smirnova GB, Borisova YuA, Treshchalina EM. Expression of receptors for α-fetoprotein in homogenates of human tumors SW620, T47D and in the culture of HEPG2 cells from the Collection of strains of human tumors of N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences. Ross. Bioter. Zh. 2016;15(1):116-117. Russian.

  9. 9.

    Cherkasova ZhR, Tsurkan SA, Smirnova GB, Borisova YuA, Treshchalina EM. Specificity of binding of Aimpila preparation with α-fetoprotein receptors on the surface of human tumor T47D from the Collection of strains of human tumors of N.N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences. Ross. Bioter. Zh. 2016; 15(1):117. Russian.

  10. 10.

    Pak V. Patent RU No. 2438695. Composition of alpha-fetoprotein and apoptosis inductors for cancer treatment. Bull. No. 1. Published January 10, 2012.

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Correspondence to E. Yu. Grigor’eva.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 164, No. 8, pp. 224-227, August, 2017

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Grigor’eva, E.Y., Treshchalina, E.M., Lipengolts, A.A. et al. Biodistribution of Alpha-Fetoprotein-Containing Noncovalent Complex Aimpila with Antitumor Activity. Bull Exp Biol Med 164, 195–198 (2017). https://doi.org/10.1007/s10517-017-3956-y

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Key Words

  • [125I]Aimpila
  • [125I]alpha-fetoprotein
  • tumor
  • xenografts
  • biodistribution