Interaction of Constitutive Nitric Oxide Synthases with Cyclooxygenases in Regulation of Bicarbonate Secretion in the Gastric Mucosa

  • V. A. ZolotarevEmail author
  • Yu. V. Andreeva
  • E. Vershinina
  • R. P. Khropycheva

Neuronal NO synthase blocker 7-nitroindazole suppressed bicarbonate secretion in rat gastric mucosa induced by mild local irritation with 1 M NaCl (pH 2.0). Non-selective blocker of neuronal and endothelial synthases, Nω-nitro-L-arginine (L-NNA), did not affect HCO3 production, but inhibited secretion after pretreatment with omeprazole. Non-selective cyclooxygenase blocker indomethacin inhibited HCO3 production under conditions of normal synthase activity and in the presence of L-NNA, but was ineffective when co-administered with 7-nitroindazole. It was concluded that neuronal and endothelial synthases are involved in different mechanisms of regulation of HCO3 secretion in the gastric mucosa induced by mild irritation. Activation of neuronal synthase stimulated HCO3 production, which is mediated mainly through activation of cyclooxygenase. Theoretically, activation of endothelial synthase should suppress HCO3 production. The effect of endothelial synthase depends on acid secretion in the stomach and bicarbonate concentration in the submucosa, as it was demonstrated in experiments with intravenous NaHCO3 infusion.

Key Words

stomach weak irritants bicarbonates endothelial and neuronal nitric oxide synthases indomethacin 


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© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • V. A. Zolotarev
    • 1
    Email author
  • Yu. V. Andreeva
    • 1
  • E. Vershinina
    • 1
  • R. P. Khropycheva
    • 1
  1. 1.I. P. Pavlov Institute of PhysiologyRussian Academy of SciencesSt. PetersburgRussia

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