Bulletin of Experimental Biology and Medicine

, Volume 162, Issue 4, pp 558–562 | Cite as

Visualization of Mesenchymal Stromal Cells in 2Dand 3D-Cultures by Scanning Electron Microscopy with Lanthanide Contrasting

  • I. A. Novikov
  • I. V. Vakhrushev
  • E. N. Antonov
  • K. N. Yarygin
  • A. M. SubbotEmail author
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)

Mesenchymal stromal cells from deciduous teeth in 2D- and 3D-cultures on culture plastic, silicate glass, porous polystyrene, and experimental polylactoglycolide matrices were visualized by scanning electron microscopy with lanthanide contrasting. Supravital staining of cell cultures with a lanthanide-based dye (neodymium chloride) preserved normal cell morphology and allowed assessment of the matrix properties of the carriers. The developed approach can be used for the development of biomaterials for tissue engineering.

Key Words

scanning electron microscopy lanthanide mesenchymal stromal cells tissue engineering 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Vakhrushev IV, Antonov EN, Popova AV, Konstantinova EV, Karalkin PA, Kholodenko IV, Lupatov AY, Popov VK, Bagratashvili VN, Yarygin KN. Design of tissue engineering implants for bone tissue regeneration of the basis of new generation polylactoglycolide scaffolds and multipotent mesenchymal stem cells from human exfoliated deciduous teeth (SHED cells). Bull. Exp. Biol. Med. 2012;153(1):143-147.CrossRefPubMedGoogle Scholar
  2. 2.
    Vakhrushev IV, Suzdaltseva YG, Burunova VV, Karalkin PA, Lupatov AY, Yarygin KN. Mesenchymal cells of the decidual tooth pulp: cytophenotype and initial evaluation of possibility of their use in bone tissue engineering. Bull. Exp. Biol. Med. 2010;149(1):161-166.CrossRefPubMedGoogle Scholar
  3. 3.
    Novikov IA, Subbot AM, Fedorov AA, Griboedova IG, Antonov EN, Vakhrushev IV. Supravital lanthanoid staining for scanning electron microscopy of biological objects. Geny Kletki. 2015;10(2):90-96. Russian.Google Scholar
  4. 4.
    Al-Asadi AS, Zhang J, Li J, Potyrailo RA, Kolmakov A. Design and application of variable temperature setup for scanning electron microscopy in gases and liquids at ambient conditions. Microsc. Microanal. 2015;21(3):765-770.CrossRefPubMedGoogle Scholar
  5. 5.
    Antonov EN, Popov VK, Barinov SM, Komlev VS, Fedotov AY, Vakhrushev IV, Yarygin KN. Selective laser sintering of bioactive composite matrices for bone tissue engineering. Inorganic Materials: Applied Research. 2015;6(2):171-178.CrossRefGoogle Scholar
  6. 6.
    Knight E, Murray B, Carnachan R, Przyborski S. Alvetex(R): polystyrene scaffold technology for routine three dimensional cell culture. Methods Mol. Biol. 2011;695:323-340.CrossRefPubMedGoogle Scholar
  7. 7.
    Neofytou EA, Chang E, Patlola B, Joubert LM, Rajadas J, Gambhir SS, Cheng Z, Robbins RC, Beygui RE. Adipose tissue-derived stem cells display a proangiogenic phenotype on 3D scaffolds. J. Biomed. Mater. Res. A. 2011;98(3):383-393.CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Potdar PD, Jethmalani YD. Human dental pulp stem cells: Applications in future regenerative medicine. World J. Stem Cells. 2015;7(5):839-851.CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Smith I, Silveirinha V, Stein JL, de la Torre-Ubieta L, Farrimond JA, Williamson EM, Whalley BJ. Human neural stem cell-derived cultures in three-dimensional substrates form spontaneously functional neuronal networks. J. Tissue Eng. Regen. Med. 2015. doi:  10.1002/term.2001.PubMedCentralGoogle Scholar
  10. 10.
    Su WT, Wu PS, Ko CS, Huang TY. Osteogenic differentiation and mineralization of human exfoliated deciduous teeth stem cells on modified chitosan scaffold. Mater. Sci. Eng. C. Mater. Biol. Appl. 2014;41:152-160.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • I. A. Novikov
    • 1
  • I. V. Vakhrushev
    • 2
  • E. N. Antonov
    • 3
  • K. N. Yarygin
    • 2
  • A. M. Subbot
    • 1
    Email author
  1. 1.Institute of Eye DiseasesMoscowRussia
  2. 2.V. N. Orekhovich Research Institute of Biomedical ChemistryMoscowRussia
  3. 3.Institute on Laser and Information TechnologiesRussian Academy of SciencesMoscowRussia

Personalised recommendations