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Bulletin of Experimental Biology and Medicine

, Volume 162, Issue 1, pp 98–101 | Cite as

Stimulation of Diethylnitrosamine Metabolism Reduces Its General Toxic and Hepatocarcinogenic Effects

  • S. I. Il’nitskaya
  • V. I. Kaledin
  • L. A. Bogdanova
  • T. S. Morozkova
  • V. I. Kapustina
  • M. L. Perepechaeva
  • A. Yu. Grishanova
Article

The general toxic and hepatocarcinogenic effects of diethylnitrosamine after stimulation of its metabolism with 1,4-bis[2-(3,5-dichloropyridyloxy)]-benzene (TCPOBOP) were studied. The hydroxylating activity of liver microsomes of C57Bl/6Mv mice towards p-nitrophenol increased more than 4-fold 3 days after injection of TCPOBOP. Injection of diethylnitrosamine 3 days after TCPOBOP caused a lesser body weight loss and decrease of food consumption in C57Bl/6Mv mice than in response to diethylnitrosamine without preinduction. Injection of diethylnitrosamine to suckling ICR mice after TCPOBOP induction of cytochrome P450 2e1 activity led to development of 2-fold lesser number of tumors and pretumorous nodes in the liver in comparison with animals injected with diethylnitrosamine without induction. These data indicated that metabolism stimulation reduced the general toxic and hepatocarcinogenic effects of diethylnitrosamine.

Key Words

hepatocarcinogenesis diethylnitrosamine toxicity cytochrome P450 2e1 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • S. I. Il’nitskaya
    • 1
  • V. I. Kaledin
    • 1
  • L. A. Bogdanova
    • 2
  • T. S. Morozkova
    • 2
  • V. I. Kapustina
    • 2
  • M. L. Perepechaeva
    • 3
  • A. Yu. Grishanova
    • 3
  1. 1.Institute of Cytology and Genetics, Siberian Division of the Russian Academy of SciencesNovosibirskRussia
  2. 2.Institute of Molecular Pathology and PathomorphologyNovosibirskRussia
  3. 3.Research Institute of Molecular Biology and BiophysicsNovosibirskRussia

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