Bulletin of Experimental Biology and Medicine

, Volume 161, Issue 2, pp 248–251 | Cite as

Radioprotective and Apoptotic Properties of a Combination of α-Tocopherol Acetate and Ascorbic Acid

  • I. N. Vasil’eva
  • V. G. Bespalov
  • D. A. Baranenko
Article

We studied radioprotective and apoptotic properties of a combination of α-tocopherol acetate and ascorbic acid. α-Tocopherol acetate (10 mg/kg body weight) or ascorbic acid (20 mg/kg) or combination of these agents in the same doses was orally administered to male rats at various terms before and after single whole-body exposure to γ-irradiation in the doses of 2 and 8 Gy. Irradiation increased the frequency of chromosome aberrations in bone marrow cells and plasma level of low-molecular-weight DNA. Vitamin combination administered before or after irradiation significantly reduced the frequency of chromosome aberrations by 2-2.5 times. Administration of this combination 10 min before irradiation 1.5-fold increased the content of low-molecular-weight DNA in blood plasma in comparison with the control animals exposed to radiation. The combination of α-tocopherol acetate and ascorbic acid produced radioprotective effects and enhanced apoptosis in irradiated cells.

Key Words

α-tocopherol acetate ascorbic acid radioprotective substances low-molecularweight DNA apoptosis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    I. N. Vasilyeva and V. G. Bespalov, Role of extracellular DNA in the appearance and development of malignant tumors and possibilities of its use in the diagnosis and treatment of cancer. Voprosy Onkol., 59, No. 6, 673-681 (2013).Google Scholar
  2. 2.
    I. N. Vasilyeva, O. I. Podgornaya, and V. G. Bespalov, Ucleosome fracton of extracellular DNA as the index of apoptosis. Tsitologiya, 57, No. 2, 87-94 (2015).Google Scholar
  3. 3.
    V. N. Zinkin, I. N. Vasilieva, and I. A. Vozniuk, Assay of extracellular low-molecular DNA in blood as a diagnostic technique for clinical and experimental studies. Aviakosmich., Ekologich. Meditsina, 45, No. 5, 47-51 (2011).Google Scholar
  4. 4.
    Krasil’nikov I.I., Zhakovko E.B., Patent RF No. 2179020. Radioprotective Agent, Bull., No. 4 (February 10, 2002).Google Scholar
  5. 5.
    A. V. Sevan’kaev, E. V. Goloub, I. K. Khvostunov, O. I. Potetnya, T. G. Shkavrova, V. G. Skvortsov, A. I. Ivannikov, D. D. Tikunov, O. S. Sidorov, G. N. Amiev, B. M. Emel’yanenko, Ph. Voisin, V. Durand, and L. Roy, Retrospective Dose Estimation in Remote Period after Exposure Using Different Biological Methods. Radiats. Biol. Radioekol., 44, No. 6, 637-652 (2004).Google Scholar
  6. 6.
    L. Garcia-Quevedo, J. Blanco, Z. Sarrate, and F. Vidal, Apoptosis mediated by phosphatidylserine externalization in the\elimination of aneuploid germ cells during human spermatogenesis. Andrology, 2, No. 6, 892-898 (2014).CrossRefPubMedGoogle Scholar
  7. 7.
    D. R. Green, Apoptotic pathways: ten minutes to dead. Cell, 121, No. 5, 671-674 (2005).CrossRefPubMedGoogle Scholar
  8. 8.
    V. K. Singh, P. K. Singh, S. Y. Wise, A. Posarac, and O. O. Fatanmi, Radioprotective properties of tocopherol succinate against ionizing radiation in mice. J. Radiat. Res., 54, No. 2, 210-220 (2013).CrossRefPubMedGoogle Scholar
  9. 9.
    C. O. Wambi, J. K. Sanzari, C. M. Sayers, M. Nuth, Z. Zhou, J. Davis, N. Finnberg, J. S. Lewis-Wambi, J. H. Ware, W. S. El-Deiry, and A. R. Kennedy, Protective effects of dietary antioxidants on proton total-body irradiation-mediated hematopoietic cell and animal survival. Radiat. Res., 172, No. 2, 175-186 (2009).CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    H. Zhang, S. B. Zhang, W. Sun, S. Yang, M. Zhang, W. Wang, C. Liu, K. Zhang, S. Swarts, B. M. Fenton, P. Keng, D. Maguire, P. Okunieff, L. Zhang, B1 sequence-based real-time quantitative PCR: a sensitive method for direct measurement of mouse plasma DNA levels after gamma irradiation. Int. J. Radiat. Oncol. Biol. Phys., 74, No. 5, 1592-1599 (2009).CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • I. N. Vasil’eva
    • 1
    • 2
  • V. G. Bespalov
    • 1
    • 2
  • D. A. Baranenko
    • 2
  1. 1.Laboratory of Chemoprevention of Cancer and Oncopharmacology, N. N. Petrov Institute of Oncology, Ministry of the Healthcare of the Russian FederationSt. PetersburgRussia
  2. 2.International Scientific Center Biotechnologies of the Third Millennium, University of Informational Technologies, Mechanics and OpticsSt. PetersburgRussia

Personalised recommendations