Maintenance of High Cytochrome P450 Expression in HepaRG Cell Spheroids in DMSO-Free Medium

  • A. V. AleksandrovaEmail author
  • O. A. Burmistrova
  • K. A. Fomicheva
  • D. A. Sakharov

We studied the effects of DMSO and fibroblasts during HepaRG cell spheroid formation and conditions of their subsequent culturing on the levels of mRNA of the major cytochromes P450. A protocol of spheroid formation from differentiated HepaRG cells and their culturing in serum- and DMSO-free medium is developed.

Key Words

HepaRG differentiation cytochromes P450 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • A. V. Aleksandrova
    • 1
    Email author
  • O. A. Burmistrova
    • 1
  • K. A. Fomicheva
    • 2
  • D. A. Sakharov
    • 1
    • 3
  1. 1.BioClinicum Research and Production CenterMoscowRussia
  2. 2.National Medical Research Radiology CenterMoscowRussia
  3. 3.Moscow State University of Mechanical Engineering (MAMI)MoscowRussia

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