Bulletin of Experimental Biology and Medicine

, Volume 157, Issue 2, pp 249–252

Biological Effects of Individually Synthesized TNF-Binding Domain of Variola Virus CrmB Protein

  • D. D. Tsyrendorzhiev
  • I. A. Orlovskaya
  • S. V. Sennikov
  • T. V. Tregubchak
  • I. P. Gileva
  • M. D. Tsyrendorzhieva
  • S. N. Shchelkunov
Virology

The biological characteristics of a 17-kDa protein synthesized in bacterial cells, a TNF-binding domain (VARV-TNF-BP) of a 47-kDa variola virus CrmB protein (VARV-CrmB) consisting of TNF-binding and chemokine-binding domains, were studied. Removal of the C-terminal chemokine-binding domain from VARV-CrmB protein was inessential for the effi ciency of its inhibition of TNF cytotoxicity towards L929 mouse fi broblast culture and for TNF-induced oxidative metabolic activity of mouse blood leukocytes. The results of this study could form the basis for further studies of VARV-TNF-BP mechanisms of activity for prospective use in practical medicine.

Keywords

variola virus leukocytes tumor necrosis factor-binding protein cytotoxicity oxidative metabolic function 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    I. P. Gileva, E. M. Malkova, E. S. Nepomnyashchikh, et al., Tsitokin. Vospal., 5, No. 1, 44-49 (2006).Google Scholar
  2. 2.
    I. P. Gileva, T. S. Nepomnyashchikh, I. A. Ryazankin, and S. N. Shchelkunov, Biokhimiya, 74, No. 12, 1664-1671 (2009).Google Scholar
  3. 3.
    I.P. Gileva, I. A. Ryazankin, T. S. Nepomnyashchikh, et al., Mol. Biol., 39, No. 2, 245-254 (2005).CrossRefGoogle Scholar
  4. 4.
    L. R. Lebedev, I. A. Ryazankin, A. A. Sizov, et al., Biotekhnol., No. 6, 14-18 (2001).Google Scholar
  5. 5.
    T. S. Nepomnyashchikh, D. V. Antonets, I. P. Gileva, and S. N. Shchelkunov, Uspekhi Sovrem. Biol., 127, No. 6, 558-569 (2007).Google Scholar
  6. 6.
    T. V. Tregubchak, T. S. Nepomnyashchikh, D. V. Antonets, and S. N. Shchelkunov, Patent RF No. 2471869. Recombinant DNA Plasmids pQE-60-TNFR-CrmB-Ind-67 and pFastBac1- G2R-dSECRET, containing Variola Virus Genome Fragment Coding for Tumor Necrosis Factor, Binding CrmB Protein Domain and Bv/G2R-dSECRET Baculovirus Strain, Producing Secretory TNF-Binding Protein of Variola Virus CrmB with Deleted Secret Domain, Bull. No. 1 (January 10, 2013).Google Scholar
  7. 7.
    D. D. Tsyrendorzhiev, S. V. Sennikov, E. A. Vyazovaya, et al., Byull. Sib. Otdel. Ross. Akad. Med. Nauk, 31, No. 3, 73-79 (2011).Google Scholar
  8. 8.
    D. D. Tsyrendorzhiev, S. V. Sennikov, I. A. Orlovskaya, et al., Immunol., 32, No. 4, 209-213 (2011).Google Scholar
  9. 9.
    A. Alejo, M. B. Ruiz-Arguello, Y. Ho, et al., Proc. Natl. Acad. Sci. USA, 103, No. 15, 5995-6000 (2006).PubMedCentralPubMedCrossRefGoogle Scholar
  10. 10.
    J. M. Bathon, R. W. Martin, R. M. Fleischmann, et al., N. Engl. J. Med., 343, No. 22, 1586-1593 (2000).PubMedCrossRefGoogle Scholar
  11. 11.
    M. M. Bradford, Anal. Biochem., 72, 248-254 (1976).PubMedCrossRefGoogle Scholar
  12. 12.
    I. P. Gileva, T. S. Nepomnyashchikh, D. V. Antonets, et al., Biochim. Biophys. Acta, 1764, No. 11, 1710-1718 (2006).PubMedCrossRefGoogle Scholar
  13. 13.
    S. A. Siegel, D. J. Shealy, M. T. Nakada, et al., Cytokine, 7, No. 1, 15-25 (1995).PubMedCrossRefGoogle Scholar
  14. 14.
    T. Tono-oka, N. Ueno, T. Matsumoto, et al., Clin. Immunol. Immunopathol., 26, No. 1, 66-75 (1983).PubMedCrossRefGoogle Scholar
  15. 15.
    M. E. Weinblatt, E. C. Keystone, D. E. Furst, et al., Arthritis Rheum., 48, No. 1, 35-45 (2003).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • D. D. Tsyrendorzhiev
    • 1
    • 2
  • I. A. Orlovskaya
    • 1
  • S. V. Sennikov
    • 1
  • T. V. Tregubchak
    • 3
  • I. P. Gileva
    • 3
  • M. D. Tsyrendorzhieva
    • 1
    • 2
  • S. N. Shchelkunov
    • 3
    • 4
  1. 1.Research Institute of Clinical ImmunologySiberian Division of the Russian Academy of Medical SciencesNovosibirskRussia
  2. 2.Novosibirsk State Medical UniversityMinistry of Health and Social Development of the Russian FederationNovosibirskRussia
  3. 3.Vector State Research CenterKol’tsovo, Novosibirsk RegionNovosibirskRussia
  4. 4.Institute of Cytology and GeneticsSiberian Division of the Russian Academy of SciencesNovosibirskRussia

Personalised recommendations