Bulletin of Experimental Biology and Medicine

, Volume 156, Issue 5, pp 658–662 | Cite as

Effect of Peptides Corresponding to Extracellular Domains of Serotonin 1B/1D Receptors and Melanocortin 3 and 4 Receptors on Hormonal Regulation of Adenylate Cyclase in Rat Brain


The ligand-recognizing part of G protein-coupled receptors consists of their extracellular loops and N-terminal domain. Identifi cation of these sites is essential for receptor mapping and for the development and testing of new hormone system regulators. The peptides corresponding by their structure to extracellular loop 2 of serotonin 1B/1D receptor (peptide 1), extracellular loop 3 of melanocortin 3 receptor (peptide 2), and N-terminal domain of melanocortin 4 (peptide 3) were synthesized by the solid-phase method. In synaptosomal membranes isolated from rat brain, peptide 1 (10–5-10–4 M) attenuated the effects of 5-nonyloxytryptamine (selective agonist of serotonin 1B/1D receptor) and to a lesser extent serotonin and 5-methoxy-N,N-dimethyltryptamine acting on all the subtypes of serotonin receptor 1. Peptide 2 (10–5-10–4 M) signifi cantly reduced the adenylate cyclase-stimulating effect of γ-melanocyte-stimulating hormone (agonist of melanocortin receptor 3), but had no effect on the adenylate cyclase effect of THIQ (agonist melanocortin receptor 4). Peptide 3 reduced the adenylate cyclase-stimulating effects of THIQ and α-melanocyte-stimulating hormone (non-selective agonist of melanocortin receptors 3 and 4), but did not modulate the effect of γ-melanocyte-stimulating hormone. The effect of peptide 3 was weaker: it was observed at peptide 3 concentration of 10–4 M. Peptides 1-3 did no change the adenylate cyclase-modulating effects of hormones acting through non-homologous receptors. Thus, the synthesized peptides specifi cally inhibited the regulatory effects of hormones acting through homologous receptors. This suggests that the corresponding extracellular domains are involved in ligand recognition and binding and determine functional activity of the receptor.

Key Words

adenylate cyclase extracellular loop melanocortin receptor peptide serotonin receptor 


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • E. A. Shpakova
    • 1
  • K. V. Derkach
    • 1
  • A. O. Shpakov
    • 1
  1. 1.Laboratory of Molecular Endocrinology, I. M. Sechenov Institute of Evolution Physiology and BiochemistryRussian Academy of SciencesSt. PetersburgRussia

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