Bulletin of Experimental Biology and Medicine

, Volume 155, Issue 1, pp 122–128

Characteristics of Induced Human Pluripotent Stem Cells Using DNA Microarray Technology

  • S. P. Medvedev
  • M. A. Smetanina
  • A. I. Shevchenko
  • I. S. Zakharova
  • A. A. Malakhova
  • E. V. Grigor’eva
  • E. V. Dementyeva
  • M. A. Aleksandrova
  • R. A. Poltavtseva
  • V. N. Veriasov
  • M. L. Filipenko
  • G. T. Sukhikh
  • E. A. Pokushalov
  • S. M. Zakian
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)

DOI: 10.1007/s10517-013-2096-2

Cite this article as:
Medvedev, S.P., Smetanina, M.A., Shevchenko, A.I. et al. Bull Exp Biol Med (2013) 155: 122. doi:10.1007/s10517-013-2096-2

We performed transcriptome analysis of some human induced pluripotent stem cells, embryonic stem cells, and human somatic cells using DNA microarrays. PluriTest bioinformatic system was used for evaluation of cell pluripotency. Changes in the genome structure and status of X-chromosome gene expression was analyzed using microarray technology.

Keywords

induced pluripotent stem cells DNA-microarray transcriptome chromosome Rearrangements 

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • S. P. Medvedev
    • 1
    • 2
    • 3
  • M. A. Smetanina
    • 2
  • A. I. Shevchenko
    • 1
    • 2
    • 3
  • I. S. Zakharova
    • 1
    • 2
    • 3
  • A. A. Malakhova
    • 1
    • 2
    • 3
  • E. V. Grigor’eva
    • 1
    • 2
    • 3
  • E. V. Dementyeva
    • 1
    • 2
    • 3
  • M. A. Aleksandrova
    • 4
  • R. A. Poltavtseva
    • 5
  • V. N. Veriasov
    • 6
  • M. L. Filipenko
    • 2
  • G. T. Sukhikh
    • 6
  • E. A. Pokushalov
    • 3
  • S. M. Zakian
    • 1
    • 2
    • 3
  1. 1.Institute of Cytology and GeneticsSiberian Division of the Russian Academy of SciencesSiberiaRussia
  2. 2.Institute of Chemical Biology and Fundamental MedicineSiberian Division of the Russian Academy of SciencesNovosibirskRussia
  3. 3.E. N. Meshalkin Research Institute of Circulatory PathologyMinistry of Health and Social DevelopmentNovosibirskRussia
  4. 4.N. K. Kol’tsov Institute of Developmental BiologyRussian Academy of SciencesMoscowRussia
  5. 5.Institute of Theoretical and Experimental BiophysicsRussian Academy of SciencesNovosibirskRussia
  6. 6.V. I. Kulakov Research Center of Obstetrics, Gynecology, and PerinatologyMinistry of Health Care and Social Development of the Russian FederationMoscowRussia

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