Cloning and Expression of Human Neuron-Specific Enolase cDNA in Escherichia coli
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cDNA fragment encoding neuron-specific enolase was amplified from the cDNA library of human brain. Then the fragment was cloned for expression in E. coli using the vector pET28-a. High level of neuron-specific enolase expression was confirmed by SDS-PAAG electrophoresis and immunochemical identity by immunoblot analysis. The constructed producer strain is the cheapest source of neuron-specific enolase suitable for the use in diagnostic applications.
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neuron-specific enolase recombinant E. coli central nervous systemPreview
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