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Neurons and Stromal Stem Cells as Targets for Polycation-Mediated Transfection

  • A. V. ShakhbazauEmail author
  • D. G. Shcharbin
  • N. V. Goncharova
  • I. N. Seviaryn
  • S. M. Kosmacheva
  • N. A. Kartel
  • M. Bryszewska
  • J. P. Majoral
  • M. P. Potapnev
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)

Expression of transgenes in neurons and stromal/mesenchymal stem cells (MSC) can greatly enhance their therapeutic potential. In transfection experiments, we studied properties of linear and branched (dendrimers) polycations as transgene delivery vehicles. Linear polyethyleneimine transfected neurons, but was ineffective in MSC. Polyamidoamine dendrimers showed greater transfection efficiency and mean GFP fluorescence intensity compared to phosphorus dendrimers of the same (4th) generation. Expression of neurotrophic factor BDNF in MSC transfected with polyamidoamine dendrimers was also by more than 10 times higher.

Key Words

neurons stromal/mesenchymal stem cells transfection polyethylenimine dendrimers brain-derived neurotrophic factor 

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Copyright information

© Springer Science+Business Media, Inc. 2011

Authors and Affiliations

  • A. V. Shakhbazau
    • 1
    • 2
    Email author
  • D. G. Shcharbin
    • 3
  • N. V. Goncharova
    • 4
  • I. N. Seviaryn
    • 4
  • S. M. Kosmacheva
    • 4
  • N. A. Kartel
    • 2
  • M. Bryszewska
    • 5
  • J. P. Majoral
    • 6
  • M. P. Potapnev
    • 4
  1. 1.Department of Clinical NeurobiologyUniversity of CalgaryCalgaryCanada
  2. 2.Institute of Genetics and CytologyNational Academy of Sciences of BelarusMinskBelarus
  3. 3.Institute of Biophysics and Cell Engineering of National Academy of Sciences of BelarusMinskBelarus
  4. 4.Russian Research-and-Production Center of Hematology and TransfusiologyMinskBelarus
  5. 5.Department of General BiophysicsUniversity of LodzLodzPoland
  6. 6.Laboratory of Coordination ChemistryNational Center for Scientific Research of FranceToulouseFrance

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