Optimization of a Method for Preparation and Repopulation of the Tracheal Matrix for Allogenic Transplantation

  • M. V. Kiselevsky
  • N. Yu. Anisimova
  • O. V. Lebedinskaya
  • B. E. Polotskii
  • M. I. Davydov
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
First Online:
Received:

A method for decellularization of the trachea for subsequent repopulation with allogenic MSC was optimized. Tracheas from C57BL/6 mice were devitalized and repopulated with MSC from BALB/c mice. The tracheal matrix with devitalized mucosa and intact cartilaginous structure, fit for repopulation with allogenic MSC, was obtained by chemical treatment with NaClO4. This approach seemed to be promising for transplantation of allogenic trachea.

Key Words

transplantation trachea mesenchymal stem cells 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    D. Campioni, F. Lanza, S. Moretti, et al., Cytotherapy, 5, No. 1, 566–759 (2003).Google Scholar
  2. 2.
    P. Carbognani, L. Spaggiari, P. Solli, et al., Eur. Surg. Res., 31, No. 2, 210–215 (1999).PubMedCrossRefGoogle Scholar
  3. 3.
    H. C. Grillo, Ann. Thorac. Surg., 73, No. 6, 1995–2004 (2002).PubMedCrossRefGoogle Scholar
  4. 4.
    P. Jungebluth, T. Go, A. Asnaghi, et al., J. Thorac. Cardiovasc. Surg., 138, No. 3, 586–593 (2009).PubMedCrossRefGoogle Scholar
  5. 5.
    K. Kojima, L. J. Bonassar, A. K. Roy, et al., Ibid., 123, No. 6, 1177–1184 (2002).Google Scholar
  6. 6.
    P. Macchiarini, P. Jungebluth, T. Go, et al., Lancet, 372, 2023–2030 (2008).PubMedCrossRefGoogle Scholar
  7. 7.
    T. Murakawa, J. Nakajima, N. Motomura, et al., J. Thorac. Cardiovasc. Surg., 123, No. 1, 153–160 (2002).PubMedCrossRefGoogle Scholar
  8. 8.
    T. Murakawa, J. Nakajima, M. Ono, et al., Ibid., 120, No. 4, 712–719 (2000).Google Scholar
  9. 9.
    J. Nakajima, M. Ono, J. Kobayashi, et al., Transplant. Proc., 30, No. 7, 3366–3367 (1998).PubMedCrossRefGoogle Scholar
  10. 10.
    R. Nakanishi, M. Hashimoto, H. Muranaka, and K. Yasumoto, J. Heart Lung Transplant., 20, No. 9, 1010–1015 (2001).PubMedCrossRefGoogle Scholar
  11. 11.
    N. T. Remlinger, C. A. Czajka, and M. E. Juhas, Biomaterials, 31, 3520–3526 (2010).PubMedCrossRefGoogle Scholar
  12. 12.
    T. Sato and T. Nakamura, Lancet, 372, 2003–2004 (2008).PubMedCrossRefGoogle Scholar
  13. 13.
    M. Sykes, N. Engl. J. Med., 362, No. 2, 172–174 (2010).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2011

Authors and Affiliations

  • M. V. Kiselevsky
    • 1
  • N. Yu. Anisimova
    • 1
  • O. V. Lebedinskaya
    • 1
  • B. E. Polotskii
    • 1
  • M. I. Davydov
    • 1
  1. 1.N. N. Blokhin Russian Oncological Research CenterRussian Academy of Medical SciencesMoscowRussia

Personalised recommendations