Bulletin of Experimental Biology and Medicine

, Volume 148, Issue 4, pp 692–696

Cardiomyocyte Regeneration in Pericicatricial Zone of the Myocardium after Laser Tunneling and Implantation of Bone Marrow Mononuclear Cells

  • P. M. Larionov
  • A. M. Chernyavskii
  • I. V. Kuznetsova
  • E. L. Lushnikova
  • V. Yu. Bondar’
  • E. V. Uglova
  • A. V. Bocharova
  • O. A. Subbotina
  • L. M. Nepomnyashchikh
  • A. M. Karas’kov
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)

DOI: 10.1007/s10517-010-0796-4

Cite this article as:
Larionov, P.M., Chernyavskii, A.M., Kuznetsova, I.V. et al. Bull Exp Biol Med (2009) 148: 692. doi:10.1007/s10517-010-0796-4

Using canine model of chronic postinfarction myocardial ischemia we evaluated angiogenesis and cardiomyogenesis after combined revascularization by laser tunneling of the pericicatricial zone followed by implantation of bone marrow mononuclear cells into the channels. It was shown that the numerical density of arterioles and capillaries considerably increased 1 month after revascularization. We also observed a considerable increase in the density of cardiomyocyte nuclei in the test area, a 2-fold increase in the total area of nuclei, and an increase in the mean area of cardiomyocyte nuclei. The number of PNCA-positive cardiomyocytes signifi cantly increased after combined revascularization. Our fi ndings suggest that combined revascularization of the pericicatricial zone of the myocardium promoted angiogenesis and stimulates regeneration of cardiomyocytes.

Key Words

revascularization of the myocardium microcirculation regeneration of cardiomyocytes 

Copyright information

© Springer Science+Business Media, Inc. 2009

Authors and Affiliations

  • P. M. Larionov
    • 1
  • A. M. Chernyavskii
    • 1
  • I. V. Kuznetsova
    • 1
  • E. L. Lushnikova
    • 2
  • V. Yu. Bondar’
    • 1
  • E. V. Uglova
    • 1
  • A. V. Bocharova
    • 1
  • O. A. Subbotina
    • 2
  • L. M. Nepomnyashchikh
    • 2
  • A. M. Karas’kov
    • 2
  1. 1.E. N. Meshalkin Research Institute of Circulatory Pathology, Federal Agency for High-Technological Medical CareNovosibirskRussia
  2. 2.Research Institute of Regional Pathology and Pathomorphology, Siberian Division of Russian Academy of Medical SciencesNovosibirskRussia

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