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Spontaneous Aneuploidy and Clone Formation in Adipose Tissue Stem Cells during Different Periods of Culturing

  • O. A. Buyanovskaya
  • N. P. Kuleshov
  • V. A. Nikitina
  • E. S. Voronina
  • L. D. Katosova
  • N. P. Bochkov
Cell Technologies in Biology and Medicine

Cytogenetic analysis of 13 mesenchymal stem cell cultures isolated from normal human adipose tissue was carried out at different stages of culturing. The incidence of chromosomes 6, 8, 11, and X aneuploidy and polyploidy was studied by fl uorescent in situ hybridization. During the early passages, monosomal cells were more often detected than trisomal ones. A clone with chromosome 6 monosomy was detected in three cultures during late passages.

Key Words

stem cells aneuploidy clone formation interphase nuclei fl uorescent in situ hybridization (FISH) 

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Copyright information

© Springer Science+Business Media, Inc. 2009

Authors and Affiliations

  • O. A. Buyanovskaya
    • 1
  • N. P. Kuleshov
    • 1
  • V. A. Nikitina
    • 2
  • E. S. Voronina
    • 2
  • L. D. Katosova
    • 2
  • N. P. Bochkov
    • 1
  1. 1.M. Sechenov Moscow Medical AcademyMoscowRussia
  2. 2.Medical Genetic CenterRussian Academy of Medical SciencesMoscowRussia

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