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Bulletin of Experimental Biology and Medicine

, Volume 148, Issue 1, pp 103–105 | Cite as

Preparation of Mixed Keratinocyte and Melanocyte Cultures from Biopsy Specimens of Pigmented Skin Sites of Patients with Vitiligo

  • I. A. Usovetskii
  • V. V. BurunovaEmail author
  • N. E. Kovtun
  • Yu. G. Suzdal’tseva
  • Yu. B. Krasil’nikova
  • N. G. Korotkii
  • K. N. Yarygin
Methods
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A method for preparation and expansion of mixed keratinocyte and melanocyte culture from human skin biopsy specimens was developed. The culture contains two melanocyte types: derivatives of hair follicle stem cells and epidermal basal layer stem cells. Fibroblasts were completely eliminated after culturing in selective medium.

Key Words

melanocytes keratinocytes vitiligo 

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Copyright information

© Springer Science+Business Media, Inc. 2009

Authors and Affiliations

  • I. A. Usovetskii
    • 1
  • V. V. Burunova
    • 1
    Email author
  • N. E. Kovtun
    • 2
    • 3
  • Yu. G. Suzdal’tseva
    • 1
  • Yu. B. Krasil’nikova
    • 1
  • N. G. Korotkii
    • 1
  • K. N. Yarygin
    • 1
    • 2
    • 3
  1. 1.Russian State Medical UniversityMoscowRussia
  2. 2.V. N. Orekhovich Institute of Biomedical ChemistryRussian Academy of Medical SciencesMoscowRussia
  3. 3.Institute of General Pathology and PathophysiologyRussian Academy of Medical SciencesMoscowRussia

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