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Activation of α-Latrotoxin Receptors in Neuromuscular Synapses Leads to a Prolonged Splash Acetylcholine Release

  • V. G. Lelyanova
  • D. Thomson
  • R. R. Ribchester
  • E. A. TonevitskyEmail author
  • Y. A. Ushkaryov
Biophysics and Biochemistry

The mechanisms of acetylcholine release in presynaptic terminals of motoneurons induced by mutant α-latrotoxin (LTN4C) were analyzed. In contrast to wild-type α-latrotoxin that causes both continuous and splash secretion of acetylcholine and necessarity block neuromuscular transmission, LTN4C causes only splash release lasting over many hours. Thus, activation of α-latrotoxin receptors controls long-lasting enhanced secretion of acetylcholine.

Key Words

neuromuscular transmission acetylcholine release latrophilin latrotoxin 

References

  1. 1.
    A. C. Ashton, M. A. Rahman, K. E. Volynski, et al., Biochimie, 82, No. 5, 453-468 (2000).CrossRefPubMedGoogle Scholar
  2. 2.
    M. Capogna, K. E. Volynski, N. J. Emptage, and Y. A. Ushkaryov, J. Neurosci., 23, No. 10, 4044-4053 (2003).PubMedGoogle Scholar
  3. 3.
    K. Ichtchenko, M. Khvotchev, N. Kiyatkin, et al., EMBO J., 17, No. 21, 6188-6199 (1998).CrossRefPubMedGoogle Scholar
  4. 4.
    E. V. Orlova, M. A. Rahman, B. Gowen, et al., Nat. Struct. Biol., 7, No. 1, 48-53 (2000).CrossRefPubMedGoogle Scholar
  5. 5.
    L. Rosenthal and J. Meldolesi, Pharmacol. Ther., 42, No. 1, 115-134 (1989).CrossRefPubMedGoogle Scholar
  6. 6.
    Y. Ushkaryov, Toxicon, 40, No. 1, 1-5 (2002).CrossRefPubMedGoogle Scholar
  7. 7.
    Y. Ushkaryov, A. Rohou, and S. Siguta, Handb. Exp. Pharmacol., No. 184, 171-206 (2008).Google Scholar
  8. 8.
    Y. A. Ushkaryov, K. E. Volynski, and A. C. Ashton, Toxicon, 43, No. 5, 527-542 (2004).CrossRefPubMedGoogle Scholar
  9. 9.
    K. E. Volynski, M. Capogna, A. C. Ashton, et al., J. Biol. Chem., 278, No. 33, 31,058-31,066 (2003).CrossRefGoogle Scholar
  10. 10.
    K. E. Volynski, E. D. Nosyreva, Y. Ushkaryov, and E. V. Grishin, FEBS Lett., 442, No. 1, 25-28 (1999).CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2009

Authors and Affiliations

  • V. G. Lelyanova
    • 1
  • D. Thomson
    • 2
  • R. R. Ribchester
    • 2
  • E. A. Tonevitsky
    • 3
    Email author
  • Y. A. Ushkaryov
    • 1
  1. 1.Department of Cell and Molecular BiologyImperial CollegeLondonU.K.
  2. 2.Department of Neurosciences of Edinburg UniversityEdinburghU.K.
  3. 3.Russian Research Institute of Sport and Physical EducationMoscowRussia

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