Plasticity of Human Mesenchymal Stem Cell Phenotype and Expression Profile under Neurogenic Conditions

  • A. V. ShakhbazovEmail author
  • N. V. Goncharova
  • S. M. Kosmacheva
  • N. A. Kartel’
  • M. P. Potapnev
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)

Human bone marrow MSC cultured in neurogenic medium containing EGF and FGFb demonstrated alteration of the phenotype and expression of neuronal precursor/early neuron markers nestin and NSE. Signals of expression of neuronal and oligodendroglial markers MAP-2, dm-20, and MBP were detected after prolongation of incubation in neurogenic medium to 2 weeks. Cells with neuronal morphology were immunopositive to early neuronal marker β-III-tubulin. Replacement of neurogenic medium for α-MEM with 10% fetal calf serum induced reversion of the phenotype to that typical for human MSC. This indicates high plasticity of the phenotype and expression profile of neuronal markers in MSC cultured under neurogenic conditions or possibility of dedifferentiation of MSC reaching the stage of neuronal precursors/early neurons.

Key Words

mesenchymal stem cells neuronal differentiation 


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Copyright information

© Springer Science+Business Media, Inc. 2009

Authors and Affiliations

  • A. V. Shakhbazov
    • 1
    Email author
  • N. V. Goncharova
    • 2
  • S. M. Kosmacheva
    • 2
  • N. A. Kartel’
    • 1
  • M. P. Potapnev
    • 2
  1. 1.Institute of Cytology and GeneticsNational Academy of Sciences of BelarusMinskBelarus
  2. 2.Center of Hematology and TransfusiologyMinskBelarus

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