Analysis of Major Isoforms of Human Growth Hormone before and after Intensive Physical Exercise
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- Sakharov, D.A., Thevis, M. & Tonevitsky, A.G. Bull Exp Biol Med (2008) 146: 466. doi:10.1007/s10517-009-0335-3
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We developed a semiquantitative method for the analysis of main growth hormone isoforms. The use of immunoaffinity sorbents, two-dimensional electrophoresis, and immunoblotting allows detection of more than 90% circulating growth hormone. It was demonstrated that the proportion of growth hormone isoforms in human serum before and after strenuous exercise remained unchanged.