Bulletin of Experimental Biology and Medicine

, Volume 146, Issue 4, pp 466–469

Analysis of Major Isoforms of Human Growth Hormone before and after Intensive Physical Exercise

Sports Medicine

DOI: 10.1007/s10517-009-0335-3

Cite this article as:
Sakharov, D.A., Thevis, M. & Tonevitsky, A.G. Bull Exp Biol Med (2008) 146: 466. doi:10.1007/s10517-009-0335-3

We developed a semiquantitative method for the analysis of main growth hormone isoforms. The use of immunoaffinity sorbents, two-dimensional electrophoresis, and immunoblotting allows detection of more than 90% circulating growth hormone. It was demonstrated that the proportion of growth hormone isoforms in human serum before and after strenuous exercise remained unchanged.

Key Words

growth hormone physical activity two-dimensional electrophoresis immunoblotting 

Copyright information

© Springer Science+Business Media, Inc. 2008

Authors and Affiliations

  • D. A. Sakharov
    • 1
    • 2
  • M. Thevis
    • 1
    • 2
  • A. G. Tonevitsky
    • 1
    • 2
  1. 1.Russian Physical Training and Sports InstituteMoscowRussia
  2. 2.Institute for BiochemistryGerman Sport UniversityCologneGermany

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