Bulletin of Experimental Biology and Medicine

, Volume 146, Issue 1, pp 158–164 | Cite as

In vitro inhibitory effect of mesenchymal stem cells on zymosan-induced production of reactive oxygen species

  • A. F. Tsyb
  • V. N. Petrov
  • A. G. Konoplyannikov
  • E. V. Saypina
  • L. A. Lepechina
  • S. Sh. Kalsina
  • I. V. Semenkova
  • E. V. Agaeva
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)

In vitro chemiluminescent test showed that human bone marrow mesenchymal stem cells and conditioned media dose-dependently inhibit production of reactive oxygen species by macrophages: 50% inhibition of chemiluminescence (compared to biocontrol) was observed at 1:1 mesenchymal stem cell/macrophage ratio or after addition of 20–25% conditioned media to the incubation medium. The observed mechanism of inhibition of production of reactive oxygen forms can play an essential role in the formation of local immunosuppressive microenvironment in the organism after allogenic transplantation of mesenchymal stem cells.

Key Words

mesenchymal stem cells macrophages reactive oxygen radicals local immunosuppressive environment 

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Copyright information

© Springer Science+Business Media, Inc. 2008

Authors and Affiliations

  • A. F. Tsyb
    • 1
  • V. N. Petrov
    • 1
  • A. G. Konoplyannikov
    • 1
  • E. V. Saypina
    • 1
  • L. A. Lepechina
    • 1
  • S. Sh. Kalsina
    • 1
  • I. V. Semenkova
    • 1
  • E. V. Agaeva
    • 1
  1. 1.Medical Radiological Research CenterRussian Academy of Medical SciencesObninskRussia

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