Bulletin of Experimental Biology and Medicine

, Volume 144, Issue 1, pp 126–129 | Cite as

Cultures of hESM human embryonic stem cells: Chromosomal aberrations and karyotype stability

  • M. A. Prokhorovich
  • M. A. Lagar’kova
  • A. G. Shilov
  • T. V. Karamysheva
  • S. L. Kiselyov
  • N. B. Rubtsov
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)

Abstract

Cytogenetic analysis of karyotypes of hESM01-hESM04 human embryonic stem cells and substrains derived from these strains showed that all these strains retained normal karyotype during long-term culturing. Two substrains of embryonic stem cells with chromosome aberrations indicating clonal origin of these strains were detected. The potentialities of using analysis of chromosome variability of embryonic stem cells for evaluation of predisposition of the corresponding genotypes to the formation of chromosome abnormalities are discussed.

Key Words

human embryonic stem cells chromosomes 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    M. A. Prokhorovich, A. G. Shilov, N. B. Rubtsov, et al., Cell Cultures. Information Bulletin [in Russian], St. Petersburg (2006), No. 21, pp. 65–68.Google Scholar
  2. 2.
    J. Buzzard, N. Gough, and J. Crook, Nature Biotechnol., 22, No. 4, 381–382 (2004).CrossRefGoogle Scholar
  3. 3.
    J. Draper, K. Smith, P. Gokhale, et al., Ibid., 22, No. 1, 53–54 (2004).CrossRefGoogle Scholar
  4. 4.
    M. Imreh, K. Gertow, J. Cederval, et al., J. Cell. Biochem., 99, 508–516 (2006).PubMedCrossRefGoogle Scholar
  5. 5.
    M. A. Lagarkova, P. Y. Volchkov, A. V. Lyakisheva, et al., Cell Cycle, 5, No. 4, 416–420 (2006).PubMedGoogle Scholar
  6. 6.
    A. Maitra, D. Arking, N. Shivapurkar, et al., Nature Genet., 37, 1099–1103 (2005).PubMedCrossRefGoogle Scholar
  7. 7.
    M. Mitalipova, R. Rao, D. Hoyer, et al., Nat. Biotechnol., 23, No. 1, 19–20 (2005).PubMedCrossRefGoogle Scholar
  8. 8.
    P. Stankievicz, I. Brozek, Z. Helias-Rodzewicz, et al., Am. J. Med. Genetics, 101, 226–239 (2001).CrossRefGoogle Scholar
  9. 9.
    R. Verma and B. Arvind, Human Chromosomes. Principles and Techniques, New York (1994), pp. 72–172.Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • M. A. Prokhorovich
    • 1
    • 3
  • M. A. Lagar’kova
    • 2
  • A. G. Shilov
    • 3
  • T. V. Karamysheva
    • 3
  • S. L. Kiselyov
    • 2
  • N. B. Rubtsov
    • 1
    • 3
  1. 1.Novosibirsk State UniversityRussia
  2. 2.N. I. Vavilov Institute of General GeneticsRussian Academy of Medical SciencesRussia
  3. 3.Institute of Cytology and GeneticsSiberian Division of Russian Academy of SciencesNovosibirsk

Personalised recommendations