Bulletin of Experimental Biology and Medicine

, Volume 141, Issue 4, pp 524–529 | Cite as

Expression of regulatory T-lymphocyte phenotype in human fetal hemopoietic and lymphoid cell culture

  • N. I. Sharova
  • A. D. Donetskova
  • I. V. Dubrovina
  • G. T. Sukhikh
  • A. A. Yarilin
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)


Cells with regulatory T-cell phenotype (Treg, CD4+CD25hi) were not detected in human fetal thymus, liver, bone marrow, spleen, and among blood mononuclears of 14–28-week gestation. The cells of the majority of these fetal thymuses express Treg specific marker (FOXP3 transcription marker) gene. Culturing of fetal liver and bone marrow cells on a monolayer of thymic epithelial cells induced expression of FOXP3 gene, but induction of CD4+CD25+ membrane phenotype was detected in only 1 of 8 studied cultures (in liver and bone marrow cells). Induction of Treg differentiation is to a greater extent determined by the characteristics of hemopoietic organ cells than of thymic epithelial cells.

Key Words

regulatory T cells thymic epithelium fetal hemopoietic organs 


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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • N. I. Sharova
    • 1
  • A. D. Donetskova
    • 1
  • I. V. Dubrovina
    • 2
  • G. T. Sukhikh
    • 2
  • A. A. Yarilin
    • 1
  1. 1.Institute of ImmunologyFMBARussia
  2. 2.National Center of Obstetrics, Gynecology, and PerinatologyRussian Academy of Medical SciencesMoscow

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