Bulletin of Experimental Biology and Medicine

, Volume 140, Issue 1, pp 138–143

Mesenchymal Stem Cells from Human Bone Marrow and Adipose Tissue: Isolation, Characterization, and Differentiation Potentialities

  • Yu. A. Romanov
  • A. N. Darevskaya
  • N. V. Merzlikina
  • L. B. Buravkova
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)

DOI: 10.1007/s10517-005-0430-z

Cite this article as:
Romanov, Y.A., Darevskaya, A.N., Merzlikina, N.V. et al. Bull Exp Biol Med (2005) 140: 138. doi:10.1007/s10517-005-0430-z

Abstract

Comparative study of cultured human bone marrow and adipose tissue (lipoaspirate) mesenchymal stem cells was carried out. The main morphological parameters, proliferative activity, expression of surface and intracellular markers of these cells were characterized. Flow cytofluorometry and histological staining showed that both cell types exhibited similar expression of CD105, CD54, CD106, HLA-I markers, were positively stained for vimentin, ASMA, collagen-1, and fibronectin, but not HLA-DR, CD117, and hemopoietic cell markers. The cells underwent differentiation into adipocytes and osteoblasts under appropriate conditions of culturing. Incubation under neuroinductive conditions led to the appearance of a cell population positively stained for type III β-tubulin (neuronal differentiation marker).

Key Words

mesenchymal stem cells cell culture bone marrow lipoaspirate differentiation phenotype 

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Yu. A. Romanov
    • 1
  • A. N. Darevskaya
    • 1
  • N. V. Merzlikina
    • 2
  • L. B. Buravkova
    • 2
  1. 1.Research and Practical Laboratory of Human Stem Cells, National Complex of Cardiology Research and PracticeMinistry of Health of the Russian FederationRussia
  2. 2.Institute of Biomedical ProblemsRussian Academy of SciencesMoscowRussia

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