Oxidative Stress in Atherosclerosis and Diabetes
- 107 Downloads
We measured the content of lipid peroxides in plasma LDL from patients with chronic CHD not accompanied by hypercholesterolemia; CHD and hypercholesterolemia; type 2 diabetes mellitus and decompensation of carbohydrate metabolism; and CHD, circulatory insufficiency, and type 2 diabetes mellitus (without hypercholesterolemia). The content of lipid peroxides in LDL isolated from blood plasma by differential ultracentrifugation in a density gradient was estimated by a highly specific method with modifications (reagent Fe2+ xylene orange and triphenylphosphine as a reducing agent for organic peroxides). The content of lipid peroxides in LDL from patients was much higher than in controls (patients without coronary heart disease and diabetes). Hypercholesterolemia and diabetes can be considered as factors promoting LDL oxidation in vivo. Our results suggest that stimulation of lipid peroxidation in low-density lipoproteins during hypercholesterolemia and diabetes is associated with strong autooxidation of cholesterol and glucose during oxidative and carbonyl (aldehyde) stress, respectively. These data illustrate a possible mechanism of the progression of atherosclerosis in patients with diabetes mellitus.
Key Wordstype 2 diabetes mellitus circulatory insufficiency low-density lipoproteins free radical oxidation autooxidation of cholesterol and glucose
Unable to display preview. Download preview PDF.
- 1.N. K. Zenkov, V. Z. Lankin, and E. B. Men'shchikova, Oxidative Stress. Biochemical and Pathophysiological Aspects [in Russian], Moscow (2001).Google Scholar
- 2.A. N. Klimov and N. G. Nikul'cheva, Normal and Abnormal Metabolism of Lipids and Lipoproteins [in Russian], St. Petersburg (1999).Google Scholar
- 3.V. Z. Lankin, A. K. Tikhaze, and Yu. N. Belenkov, Kardiologiya, 40, No.7, 48–61 (2000).Google Scholar
- 4.V. Z. Lankin, A. K. Tikhaze, and Yu. N. Belenkov, Ibid., 44, No.2, 72–81 (2004).Google Scholar
- 9.W. B. Kannel and D. L. McGee, Diabetes, 241, 2035–2038 (1979).Google Scholar
- 11.V. Lankin, Free Radicals, Nitric Oxide, and Inflammation: Molecular, Biochemical, and Clinical Aspects, Amsterdam (2003), Vol. 344, pp. 8–23.Google Scholar
- 12.V. Lankin and A. Tikhaze, Ibid., pp. 218–231.Google Scholar