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Oxidative Stress in Atherosclerosis and Diabetes

  • V. Z. Lankin
  • M. O. Lisina
  • N. E. Arzamastseva
  • G. G. Konovalova
  • L. V. Nedosugova
  • A. I. Kaminnyi
  • A. K. Tikhaze
  • F. T. Ageev
  • V. V. Kukharchuk
  • Yu. N. Belenkov
Article

Abstract

We measured the content of lipid peroxides in plasma LDL from patients with chronic CHD not accompanied by hypercholesterolemia; CHD and hypercholesterolemia; type 2 diabetes mellitus and decompensation of carbohydrate metabolism; and CHD, circulatory insufficiency, and type 2 diabetes mellitus (without hypercholesterolemia). The content of lipid peroxides in LDL isolated from blood plasma by differential ultracentrifugation in a density gradient was estimated by a highly specific method with modifications (reagent Fe2+ xylene orange and triphenylphosphine as a reducing agent for organic peroxides). The content of lipid peroxides in LDL from patients was much higher than in controls (patients without coronary heart disease and diabetes). Hypercholesterolemia and diabetes can be considered as factors promoting LDL oxidation in vivo. Our results suggest that stimulation of lipid peroxidation in low-density lipoproteins during hypercholesterolemia and diabetes is associated with strong autooxidation of cholesterol and glucose during oxidative and carbonyl (aldehyde) stress, respectively. These data illustrate a possible mechanism of the progression of atherosclerosis in patients with diabetes mellitus.

Key Words

type 2 diabetes mellitus circulatory insufficiency low-density lipoproteins free radical oxidation autooxidation of cholesterol and glucose 

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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • V. Z. Lankin
    • 1
  • M. O. Lisina
    • 1
  • N. E. Arzamastseva
    • 1
  • G. G. Konovalova
    • 1
  • L. V. Nedosugova
    • 2
  • A. I. Kaminnyi
    • 1
  • A. K. Tikhaze
    • 1
  • F. T. Ageev
    • 1
  • V. V. Kukharchuk
    • 1
  • Yu. N. Belenkov
    • 1
  1. 1.Laboratory for Biochemistry of Free Radical Processes, A. L. Myasnikov Institute of Cardiology, Russian Research and Production Center of CardiologyRussian Ministry of HealthRussia
  2. 2.Department of Endocrinology and Diabetology, Faculty of Postgraduate Medical TrainingI. M. Sechenov Moscow Medical AcademyMoscowRussia

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