Aquaculture International

, Volume 28, Issue 1, pp 377–387 | Cite as

Effects of commercial superzist probiotic on growth performance and hematological and immune indices in fingerlings Acipenser baerii

  • Mir Hamed Sayed HassaniEmail author
  • Ayoub Yousefi Jourdehi
  • Ali Hosseinpour Zelti
  • Alireza Shenavar Masouleh
  • Frozan Bagherzadeh Lakani


This study was carried out to evaluate the effects of using superzist probiotic (a mixture of Lactobacillus spp., Bacillus subtilis, and Bifidobacterium bifidum) on growth performance and hematological and some immunological indices in fingerling Acipenser baerii. In total, 240 Acipenser baerii fingerlings with mean weight 10.5 ± 0.14 g−1 were stocked in 12 tanks, 20 per each tank and each treatment in triplicate. Diets were prepared by spraying slowly the mixture of 50 ml saline serum with 100, 200, and 300 mg probiotic powder per 1-kg diet to make the concentrations 1 × 106, 2 × 106, and 3 × 106 CFU g−1 of probiotic bacteria in diet. Results showed that there was a significant increase in the final weight, weight gain (WG), percentage weight gain, condition factor (CF), and specific growth rate (SGR) in fish fed PB300 treatment compared with the control at the 8th week (p < 0.05); also, the lowest feed conversion ratio (FCR) belonged to fish fed PB300 that showed a significant difference compared with fish fed control diet (p < 0.05). Except for neutrophil, lymphocyte, and hematocrit (p < 0.05), values of all hematological parameters of fish fed different concentrations of probiotic diet did not differ from the values of fish fed the control diet (p > 0.05), but there was a significant increase in lysozyme and IgM fish fed probiotic (PB300) and compliment (ACH50) (PB200 and PB300) compared with control treatment during 56 days. Therefore, results indicated that 300 mg kg−1 probiotic (Lactobacillus and Bifidobacterium) can be used as a proper probiotic in sturgeon aquaculture to enhance fish health and growth performance.


Acipenser baerii Probiotic Growth rate Hematological indices Immune system 



The authors thank the experts and directors in International Sturgeon Research Institute (Iran) for cooperation, especially Alireza Alipour, Reza Ghorbani, Ali Hoshyar, and Arash Ghorbani.

Compliance with ethical standards

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed by the authors.

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Adineh H, Jafaryan H, Sahandi J, Alizadeh M (2013) Effect of Bacillus spp. probiotic on growth and feeding performance of rainbow trout, Oncorhynchus mykiss larvae. Bulg J Veter Med 16(1):29–36Google Scholar
  2. Anderson WG, McKinley RS, Colavecchia M (1997) The use of clove oil as an anaesthetic for rainbow trout and its effect on swimming performance. N Am J Fish Manag 17:301–307Google Scholar
  3. Askarian F, Kousha A, Ringo E (2009) Isolation of lactic acid bacteria from the gastrointestinal tract of Beluga, Huso huso and Persian sturgeon, Acipenser persicus. J Appl Ichthyol 25:91–94Google Scholar
  4. Askarian F, Kousha A, Salma W, Ringo E (2011) The effect of lactic acid bacteria administration on growth, digestive enzyme activity and gut microbiota in Persian sturgeon, Acipenser persicus and beluga, Huso huso fry. Aquacult Nut 17:488–497Google Scholar
  5. Bagheri T, Hedayati SA, Yavari V, Alizadeh M, Farzanfar A (2008) Growth, survival and gut microbial load of rainbow trout, Oncorhynchus mykiss, fry given diet supplemented with probiotic during the two months of first feeding. Turk J Fish Aquat Sci 8:43–48Google Scholar
  6. Balcazar JL, De Blas I, Zarzuela-Ruiz I, Cunningham D, Vendrell D, Mu Zquiz JL (2006) The role of probiotics in aquaculture (review). Vet Microbiol 114:173–186PubMedGoogle Scholar
  7. Broch K, Pederson IE, Hogmo RO (2015) The use of probiotics in fish feed for intensive aquaculture to promote healthy guts. Inter Schol J 7:264–273Google Scholar
  8. Bronzi P, Rosenthal H, Gessner J (2011) Global sturgeon aquaculture production: an overview. J Appl Ichthyol 27:169–1675Google Scholar
  9. Burr G, Gatlin D, Ricke S (2005) Microbial ecology of the gastrointestinal tract of fish and the potential application of prebiotics and probiotics in finfish aquaculture. J World Aquacult Soc 36:425–436Google Scholar
  10. Cruz PM, Ibanez AL, Hermosillo OAM, Ramirez Saad HC (2012) Use of probiotics in aquaculture. ISRN Microbiol 2012:916845Google Scholar
  11. El-Haroun ER, Goda AS, Kabir AM, Chowdhury MA (2006) Effect of dietary probiotic Biogen supplementation as a growth promoter on growth performance and feed utilization of Nile tilapia, Oreochromis niloticus (L.). Aquac Res 37:1473–1480Google Scholar
  12. Ellis AE (1999) Immunity to bacteria in fish. Fish Shellfish Immunol 9:291–308Google Scholar
  13. Faramarzi M, Jafaryan H, Patimar R, Iranshahi F, Boloki ML, Farahi A (2011) The effects of different concentrations of probiotic Bacillus spp. and different bioencapsulation times on growth performance and survival rate of Persian sturgeon, Acipenser persicus larvae. World J Fish Mar Sci 3:145–150Google Scholar
  14. Faramarzi M, Jafaryan H, Roozbehfar R, Jafari M, Biria M (2012a) Influences of probiotic Bacilli on ammonia and urea excretion in two conditions of starvation and satiation in Persian sturgeon Acipenser persicus larvae. Glob Vet 8:185–189Google Scholar
  15. Faramarzi M, Jafaryan H, Roozbehfar R, Jafari M, Rashidi Y, Biria M (2012b) Influences of probiotic Bacilli via bioencapsulated Daphnia magna on resistance of Persian sturgeon larvae against challenge tests. Glob Vet 8:421–425Google Scholar
  16. Gatesoupe FJ (2008) Updating the importance of lactic acid bacteria in fish farming: natural occurrence and probiotic treatments. J Mol Microbiol Biotechnol 14:107–114PubMedGoogle Scholar
  17. Geng X, Dong X, Tan B, Yang Q, Chi S, Liu H, Liu X (2011) Effects of dietary chitosan and Bacillus subtilis on the growth performance, non-specific immunity and disease resistance of cobia, Rachycentron canadum. Fish Shellfish Immunol 31:400–406PubMedGoogle Scholar
  18. Ghanbari M, Rezaei M, Jami M, Nazari RM (2009) Isolation and characterization of Lactobacillus species from intestinal contents of beluga, Huso huso and Persian sturgeon, Acipenser persicus. Iran J Vet Res Shiraz Univ 10:152–157Google Scholar
  19. Gildberg A, Mikkelsen H, Sandaker E, Ringo E (1997) Probiotic effect of lactic acid bacteria in the feed on growth and survival of fry of Atlantic cod (Gadus morhua). Hydrobiology 352:279–285Google Scholar
  20. Hallajian A, Kazemi R, Yousefi Jourdehi A (2011) Effect of clove, Caryophillium aromaticus powder on anesthesia and recovery time on farmed 4 years old beluga, Huso huso. J Fish 5(2):133–140Google Scholar
  21. Harikrishnan R, Balasundaram C, Heo MS (2010) Lactobacillus sakei BK19 enriched diet enhances the immunity status and disease resistance to streptococcosis infection in kelp grouper, Epinephelus bruneus. Fish Shellfish Immunol 29:1037–1043PubMedGoogle Scholar
  22. Hoseinifar SH, Ringo E, Shenavar Masouleh A, Esteban MA (2016) Probiotic, prebiotic and symbiotic supplements in sturgeon aquaculture: a review. Rev Aquac 8:89–102Google Scholar
  23. Hung SS O, Groff JM, Lutes PB, Kofi-Fynn-Aikinis F (1998) Hepatic and intestinal histology of juvenile white sturgeon fed different carbohydrate. Aquaculture 87:349–360.Google Scholar
  24. Ibrahem MI (2015) Evolution of probiotics in aquatic world: potential effects, the current status in Egypt and recent prospective. J Adv Res 6:765–791PubMedGoogle Scholar
  25. Iranshahi F, Faramarzi M, Kiaalvandi S, Boloki ML (2011) The enhancement of growth and feeding performance of Persian sturgeon, Acipenser persicus larvae by Artemia urmiana nauplii bioencapsulated via baker’s yeast, Saccharomyces cerevisiae. J Anim Vet Adv 10:2730–2735Google Scholar
  26. Irianto A, Austin B (2002) Use of probiotics to control furunculosis in rainbow trout, Oncorhynchus mykiss (Walbaum). J Fish Dis 25:333–342Google Scholar
  27. Kesarcodi-Watson A, Haspar H, Lategan MJ, Gibson L (2008) Probiotics in aquaculture: the need, principles and mechanisms of action and screening processes. Aquaculture 274:1–14Google Scholar
  28. Kim DH, Austin B (2006) Innate immune responses in Rainbow trout, Oncorhynchus mykiss induced by probiotics. Fish Shellfish Immunol 21:513–524PubMedGoogle Scholar
  29. Kumar R, Mukherjee SC, Ranjan R, Nayak SK (2008) Enhanced innate immune parameters in Labeo Rohita (Ham.) following oral administration of Bacillus subtilis. Fish Shellfish Immunol 24:168–172PubMedGoogle Scholar
  30. Lamas J, Santos Y, Bruno DW, Toranzo AE, Anadon R (1994) Non-specific cellular responses of rainbow trout to Vibrio anguillarium and its extracellular products (ECPs). J Fish Biol 45(5):839–854Google Scholar
  31. Lara-Flores M, Olvera-Novoa MA, Guzman-Mendez BE, Lopez-Madrid W (2003) Use of the bacteria Streptococcus faecium and Lactobacillus acidophilus, and the yeast Saccharomyces cerevisiae as growth promoters in Nile tilapia ,Oreochromis niloticus. Aquaculture 216:193–201Google Scholar
  32. Lindsay GJH (1986) The significance of chitionlytic enzyme and lysozyme in rainbow trout, Salmo gairdneri defense. Aquaculture 51:169–173Google Scholar
  33. Merrifield DL, Bradley G, Baker RTM, Davies SJ (2010b) Probiotic applications for Rainbow trout, Oncorhyncus mykiss (Walbaum) II. Effects on growth performance, feed utilization, intestinal microbiota and related health criteria post-antibiotic treatment. Aquac Nutr 16:496–503Google Scholar
  34. Merrifield DL, Dimitroglou A, Foey A, Davies SJ, Baker RMT, Bogwald J, Castex M, Ringo E (2010c) The current status and future focus of probiotic and prebiotic applications for salmonids. Aquaculture 302:1–18Google Scholar
  35. Merrifield DL, Bradley G, Harper GM, Baker RTM, Munn CB, Davies SJ (2011) Assessment of the effects of vegetative and lyophilized Pediococcus acidilacticion growth, feed utilization, intestinal colonization and health parameters of rainbow trout (Oncorhynchus mykiss Walbaum). Aquac Nutr 17:73–79Google Scholar
  36. Mohapatra S, Chakraborty T, Prusty AK, Das P, Pani Prasad K, Mohanta KN (2012) Use of different microbial probiotics in the diet of rohu, Labeo rohita fingerlings: effect on growth, nutrient digestibility and retention, digestive enzyme activities and intestinal micro-flora. Aquacult Nut 1:1–11Google Scholar
  37. Nayak SK, Swain B, Mukherjee SC (2007) Effect of dietary probiotic and vitamin C on the immune response of India major carp Labeo rohita (Ham). Fish Shellfish Immunol 23:892–896PubMedGoogle Scholar
  38. Nikoskelainen S, Ouwehand A, Salminen S, Bylund G (2001) Protection of rainbow trout, Oncorynchus mykiss from frunculosis by Lactobacillus rhamnosus. Aquaculture 198:229–236Google Scholar
  39. Panigrahi A, Kiron V, Kobayashi T, Puangkaew J, Satoh S, Sugita H (2004) Immune response in the rainbow trout Oncorhynchus mykiss induced by a potential probiotic bacteria Lactobacillus rhamnosus JCM 1136. Vet Immunol Immunopathol 102:379–388PubMedGoogle Scholar
  40. Panigrahi A, Kiron V, Puangkaew J, Kobayashi T, Satoh S, Sugita H (2005) The viability of probiotic bacteria as a factor influencing the immune response in rainbow trout, Oncorhynchus mykiss. Aquaculture 243:241–254Google Scholar
  41. Panigrahi A, Kiron V, Satoh S, Hirono I, Kobayashi T, Sugita H (2007) Immune modulation and expression of cytokine genes in rainbow trout, Oncorhynchus mykiss upon probiotic feeding. Dev Comp Immunol 31:372–382PubMedGoogle Scholar
  42. Pirarat N, Pinpimai K, Endo M, Katagiri T, Ponpornpisit A, Chansue N (2011) Modulation of intestinal morphology and immunity in Nile tilapia, Oreochromis niloticus by Lactobacillus rhamnosus GG. Res Vet Sci 91(3):92–97Google Scholar
  43. Salinas I, Abelli L, Bertoni F, Picchietti S, Roque A, Furones D, Cuesta A, Meseguer J, Esteban MA (2008) Monospecies and multispecies probiotic formulations produce different systemic and local immunostimulatory effects in the gilthead Seabream, Sparus aurata (L.). Fish Shellfish Immunol 25:114–123PubMedGoogle Scholar
  44. Soltani M, Shenavar Masouleh A, Ahmadi M, Pourkazemi M, Taherimirghaed A (2015) Antibacterial activity, antibiotic susceptibility and probiotic use of lactic acid bacteria (LAB) in Persian sturgeon, Acipenser persicus. Iran J Aquat Anim Health 2(1):54–65Google Scholar
  45. Son VM, Chang C, Wu M, Guu Y, Chiu C, Cheng W (2009) Dietary administration of the probiotic, Lactobacillus plantarum, enhanced the growth, innate immune responses, and disease resistance of the grouper, Epinephelus coioides. Fish Shellfish Immunol 26(5):691–698PubMedGoogle Scholar
  46. Song Z, Wu T, Cai L, Zhang L, Zheng X (2006) Effects of dietary supplementation with Clostridium butyricumon the growth performance and humoral immune response in Miichthys miiuy. J Zhejiang Univ Sci B 7(7):596–602PubMedPubMedCentralGoogle Scholar
  47. Sun Y, Yang H, Ma R, Lin W (2010) Probiotic applications of two dominant gut Bacillus strains with antagonistic activity improved the growth performance and immune responses of grouper Epinephelus coioides. Fish Shellfish Immunol 29:803–809PubMedGoogle Scholar
  48. Talpur AD, Ikhwanuddin MHD (2012) Dietary effects of garlic, Allium sativum on haemato-immunological parameters, survival, growth, and disease resistance against Vibrio harveyi infection in Asian sea bass, Lates calcarifer (Bloch). Aquaculture 364-365:6–12Google Scholar
  49. Tukmechi A, Morshedi A, Delirezh N (2007) Changes in intestinal microflora and humoral immune response following probiotic administration rainbow trout Oncorhyncus mykiss. J Anim Vet Adv 6(10):1183–1189Google Scholar
  50. Yazdani MA, Pourkazemi M, Shakorian M, Pourali HM, Paykaran mana N, Sayed Hassani MH, Yeganeh H, Poursafar M (2010) Beluga rearing for meat and caviar production. Iranian Fisheries Research Organization (IFRO), 59 ppGoogle Scholar
  51. Young-Hyo C, Jong-Keun K, Hong-Jong K, Won-Yong K, Young-Bae K, Yong-Ha P (2001) Selection of a potential probiotic Lactobacillus strain and subsequent in-vivo studies. A V Leeuwenhoek 80:193–199Google Scholar
  52. Yousefi Jourdehi A, Sudagar M, Bahmani M, Hoseini SA, Dehghani MA, Yazdani MA (2014) Comparative study of dietary soy phytoestrogens genistein and equol effects on growth parameters and ovarian development in farmed female beluga sturgeon, Huso huso. Fish Physiol Biochem. PubMedGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Aquaculture Department of International Sturgeon Research Institute of Caspian SeaAgricultural Research Education and Extension Organization (AREEO)RashtIran

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