Advertisement

Molecular identification reveals hybrids of Mytilus coruscus × Mytilus galloprovincialis in mussel hatcheries of China

  • 41 Accesses

Abstract

Juvenile mussel trade monitoring is becoming more and more important for mussel farms of China, which is essential for the sustainable development of mussel culture. The identification of mussel hybrids by morphological analysis is unreliable, particularly between interspecific hybrids and pure species juveniles. Mytilus coruscus are the representative local mussel species for their higher market price, which is 2–5 times of M. galloprovincialis. Therefore, in the present study, we established a methodology that allows the accurate identification of M. coruscus × M. galloprovincialis hybrid and pure juveniles using both nuclear and mitochondrial primers and analysed the mussel juveniles purchased from four different seed producers in China. Nuclear primers Myti-2 used in this study were modified from the degenerated oligonucleotide primers Myti-F/R to improve the efficiency of amplification; primers YB-16S-4 derived from female type mitochondrial DNA were found to be distinct between the two species with one single peak in high-resolution melting (HRM) analysis, which was proven to be effective in maternity determination of their hybrid progenies. For samples commercialised as M. coruscus seeds of the two farms, 88.5% juveniles were identified pure M. coruscus and 11.5% were hybrids of M. coruscus♀ × M. galloprovincialis♂in Farm A; 5.6% juvenile were pure M. galloprovincialis and 94.4% were hybrids of M. galloprovincialis♀ × M. coruscus♂in Farm D. Samples from Farm B and C were proven to be pure M. coruscus. The validated new identification method will be a useful tool for the surveillance of the production and trade of the two commercially important mussel species and their hybrid seeds, thereby facilitating the sustainable development of mussel farming. Hybrids detected in Farm A and D also highlighted the importance of juvenile mussel trade monitoring in the market to avoid the commercial fraud.

This is a preview of subscription content, log in to check access.

Access options

Buy single article

Instant unlimited access to the full article PDF.

US$ 39.95

Price includes VAT for USA

Subscribe to journal

Immediate online access to all issues from 2019. Subscription will auto renew annually.

US$ 99

This is the net price. Taxes to be calculated in checkout.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

References

  1. Ab Rahim ES, Nguyen TTT, Ingram B, Riginos C, Weston KJ, Sherman CDH (2016) Species composition and hybridization of mussel species (Bivalvia: Mytilidae) in Australia. Mar Freshw Res 67:1955–1963

  2. Boscari E, Barmintseva A, Zhang S, Yue H, Li C, Shedko SV, Lieckfeldt D, Ludwig A, Wei QE, Mugue N, Congiu L (2017) Genetic identification of the caviar- producing Amur and Kaluga sturgeons revealed a high level of concealed hybridization. Food Control 82:243–250

  3. Brannock PM, Roberts MA, Hilbish TJ (2013) Ubiquitous heteroplasmy in Mytilus spp. resulting from disruption in doubly uniparental inheritance regulation. Mar Ecol Prog Ser 480:131–143

  4. Chang K, Wu J (2007) Study on artificial propagation of mussel Mytilus coruscus. South China Fish Sci 3(3):26–30 (in Chinese with English abstract)

  5. Chen XQ, Li RH, Wang CL, Mu CK, Song WW, Liu L, Shi C, Zhan PP (2018) An effective method for identification of three mussel species and their hybrids based on SNPs. Conserv Genet Resour. https://doi.org/10.1007/s12686-018-1051-y

  6. Fernández-Tajes J, Longa A, García-Gil J, Chiu YW, Huang YS, Méndez J, Lee RS (2011) Alternative PCR–RFLP methods for mussel Mytilus species identification. Eur Food Res Technol 233:791–796

  7. Hashimoto DT, Senhorini JA, Foresti F, Martínez P, Porto-Foresti F (2014) Genetic identification of F1 and post-F1 serrasalmid juvenile hybrids in Brazilian aquaculture. PLoS One 9(3):e89902. https://doi.org/10.1371/journal.pone.0089902

  8. Hilbish TJ, Mullinax A, Dolven SI, Meyer A, Koehn RK, Rawson PD (2000) Origin of the antitropical distribution pattern in marine mussels (Mytilus spp.): routes and timing of transequatorial migration. Mar Biol 136:69–77

  9. Inoue K, Waite JH, Matsuoka M, Odo S, Harayama S (1995) Interspecific variations in adhesive protein sequences of Mytilus edulis, M. galloprovincialis, and M. trossulus. Biol Bull 189(3):370–375

  10. Jilberto F, Araneda C, Larraín MA (2017) High resolution melting analysis for identification of commercially-important Mytilus species. Food Chem 229:716–720

  11. Jin YL, Li Q, Kong LF, Yu H, Zhong XX (2015) High-resolution melting (HRM) analysis: a highly sensitive alternative for the identification of commercially important Crassostrea oysters. J Mollus Stud 81:167–170

  12. Li GP, Li J, Li D (2010) Seasonal variation in nutrient composition of Mytilus coruscus from China. J Agr Food Chem 58:7831–7837

  13. Li Q, Park C, Kijima A (2002) Isolation and characterization of microsatellite loci in the Pacific abalone, Haliotis discus hannai. J Shellfish Res 21:811–815

  14. Lotfy WM, Dejong RJ, Black BS, Loker ES (2005) Specific identification of Egyptian Biomphalaria species and possible hybrids using the polymerase chain reaction based on nuclear and mitochondrial loci. Mol Cell Probes 19(1):21–25

  15. Prado FD, Hashimoto DT, Senhorini JA, Foresti F, Porto-Foresti F (2012) Detection of hybrids and genetic introgression in wild stocks of two catfsh species (Siluriformes: Pimelodidae): the impact of hatcheries in Brazil. Fish Res 125-126:300–305

  16. Skibinski DOF, Gallagher C, Beynon CM (1994) Sex-limited mitochondrial DNA transmission in the marine mussel Mytilus edulis. Genetics 138:801–809

  17. Shen W, Ye M, Wang RX, Shi G, Zhao SJ (2011) Natural distribution and ecological impacts of the alien species Mytilus galloprovincialis in the Zhoushan waters. J Oceanogr Taiwan Strait 30(2):250–256 (in Chinese with English abstract)

  18. Wang ZR (1997) Fauna Sinica. Science Press, Beijing (in Chinese)

  19. Wood AR, Turner G, Skibinski DOF, Beaumont AR (2003) Disruption of doubly uniparental inheritance of mitochondrial DNA in hybrid mussels (Mytilus edulis × M. galloprovincialis). Heredity 91:354–360

  20. Xu F, Li L, Wang J, Zhang G (2014) Use of high-resolution melting analysis for detecting hybrids between the oysters Crassostrea sikamea and Crassostrea angulata reveals bidirectional gametic compatibility. J Mollus Stud 80:435–443

  21. Ye YY (2012) Genetic structure and genetic diversity of Mytilus coruscus in the coastal waters of Southeast China Sea. Dissertation for Master’s Degree. Zhejiang Ocean University, Zhoushan. (in Chinese with English abstract)

  22. Zhang Y, Zhang Y, Li J, Xiao S, Xiang Z, Wang Z, Yan X, Yu Z (2016) Artificial interspecific backcrosses between the hybrid of female Crassostrea hongkongensis × male C. gigas and the two parental species. Aquaculture 450:95–101

  23. Zouros E, Oberhauser Ball A, Saavedra C, Freeman KR (1994) An unusual type of mitochondrial DNA inheritance in the blue mussel Mytilus. Proc Natl Acad Sci U S A 91:7463–7467

Download references

Author information

Correspondence to Ronghua Li.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Zhang, W., Li, R., Chen, X. et al. Molecular identification reveals hybrids of Mytilus coruscus × Mytilus galloprovincialis in mussel hatcheries of China. Aquacult Int 28, 85–93 (2020) doi:10.1007/s10499-019-00445-8

Download citation

Keywords

  • Mussel
  • Mytilus coruscus
  • Mytilus galloprovincialis
  • Hybrid
  • Single-nucleotide polymorphisms (SNPs)
  • High-resolution melting (HRM)