Aquaculture International

, Volume 27, Issue 6, pp 1835–1846 | Cite as

Colonization of enzymatic bacterial flora in biofloc grown shrimp Penaeus vannamei and evaluation of their beneficial effect

  • Akshaya PanigrahiEmail author
  • Palanichamy Esakkiraj
  • Sundaresan Jayashree
  • Chakrapani Saranya
  • Rashmi Ranjan Das
  • Mani Sundaram


Experiments were conducted to explore the colonization of beneficial bacteria in shrimp Penaeus vannamei grown in different sources of biofloc and clear water. Beneficial effect in terms of extracellular enzyme production and antibiofilm activity of the isolated strains was determined. Heterotrophic bacterial population were isolated by using different agar plates and resulted in isolation of 94 isolates in total. Extracellular enzyme production such as amylase, protease, lipase, cellulase, xylanase, and pectinase were screened. Antibiofilm activity of culture supernatants of enzymatic strains against pathogenic Vibrio was also determined. Out of 94 strains screened, 36 strains were found to produce amylase enzyme, 20 strains protease, 27 strains lipase, 6 strains cellulase, and 8 strains xylanase. Totally, 21 isolates selected for further identification and different species of Cobetia, Exiguobacterium, Bacillus, Marinilactibacillus, Staphyllococcus, and Novosphingobium genera from biofloc treatments were identified. In control group animals, strains of Bacillus and Exiguobacterium were isolated and identified. The genus Exiguobacterium was found common in all the different treatments and control. The result showed that shrimp grown on biofloc system allows colonizing more beneficial bacteria in gut than control. Few promising strains under Bacillus genus were found to produce all the extracellular enzymes along with antibiofilm activity.


Biofloc Shrimp Enzymatic bacteria Protease Amylase Lipase 



The authors are thankful to the director, CIBA for all his support for conducting this research activity.


This work received financial assistance extended by the Department of Biotechnology, Ministry of Science and Technology, New Delhi, India (F. No. DBT/PR11721/AAQ/3/683/2014).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethics approval

The research undertaken complies with the current animal welfare laws in India. Care and treatment of the experimental animal used in this study were in accordance with the guidelines of the CPCSEA [Committee for the Purpose of Control and Supervision of Experiments on Animals, Ministry of Environment & Forests (Animal Welfare Division), Govt. of India] on care and use of animals in scientific research. The study was undertaken with approval of statutory authorities of the Central Institute of Brackishwater Aquaculture, Chennai, India. The experimental animal Penaeus vannamei is not an endangered shrimp, the provisions of the Govt. of India’s Wildlife Protection Act of 1972 are not applicable for experiments on this fish.

Supplementary material

10499_2019_434_MOESM1_ESM.docx (311 kb)
ESM 1 (DOCX 311 kb)


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Akshaya Panigrahi
    • 1
    Email author
  • Palanichamy Esakkiraj
    • 1
  • Sundaresan Jayashree
    • 1
  • Chakrapani Saranya
    • 1
  • Rashmi Ranjan Das
    • 1
  • Mani Sundaram
    • 1
  1. 1.Crustacean Culture DivisionICAR-Central Institute of Brackishwater AquacultureChennaiIndia

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