Colonization of enzymatic bacterial flora in biofloc grown shrimp Penaeus vannamei and evaluation of their beneficial effect
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Experiments were conducted to explore the colonization of beneficial bacteria in shrimp Penaeus vannamei grown in different sources of biofloc and clear water. Beneficial effect in terms of extracellular enzyme production and antibiofilm activity of the isolated strains was determined. Heterotrophic bacterial population were isolated by using different agar plates and resulted in isolation of 94 isolates in total. Extracellular enzyme production such as amylase, protease, lipase, cellulase, xylanase, and pectinase were screened. Antibiofilm activity of culture supernatants of enzymatic strains against pathogenic Vibrio was also determined. Out of 94 strains screened, 36 strains were found to produce amylase enzyme, 20 strains protease, 27 strains lipase, 6 strains cellulase, and 8 strains xylanase. Totally, 21 isolates selected for further identification and different species of Cobetia, Exiguobacterium, Bacillus, Marinilactibacillus, Staphyllococcus, and Novosphingobium genera from biofloc treatments were identified. In control group animals, strains of Bacillus and Exiguobacterium were isolated and identified. The genus Exiguobacterium was found common in all the different treatments and control. The result showed that shrimp grown on biofloc system allows colonizing more beneficial bacteria in gut than control. Few promising strains under Bacillus genus were found to produce all the extracellular enzymes along with antibiofilm activity.
KeywordsBiofloc Shrimp Enzymatic bacteria Protease Amylase Lipase
The authors are thankful to the director, CIBA for all his support for conducting this research activity.
This work received financial assistance extended by the Department of Biotechnology, Ministry of Science and Technology, New Delhi, India (F. No. DBT/PR11721/AAQ/3/683/2014).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The research undertaken complies with the current animal welfare laws in India. Care and treatment of the experimental animal used in this study were in accordance with the guidelines of the CPCSEA [Committee for the Purpose of Control and Supervision of Experiments on Animals, Ministry of Environment & Forests (Animal Welfare Division), Govt. of India] on care and use of animals in scientific research. The study was undertaken with approval of statutory authorities of the Central Institute of Brackishwater Aquaculture, Chennai, India. The experimental animal Penaeus vannamei is not an endangered shrimp, the provisions of the Govt. of India’s Wildlife Protection Act of 1972 are not applicable for experiments on this fish.
- Banerjee G, Dan SK, Ankita N, Pinki G, Ray AK (2015) Autochthonous gut bacteria in two Indian air-breathing fish, climbing perch (Anabas testudineus) and walking catfish (Clarias batrachus): mode of association, identification, and enzyme producing ability. Pol J Microbiol 64(4):361–368CrossRefGoogle Scholar
- Otta SK, Praveena PE, Raj RA, Saravanan P, Priya MS, Amarnath CB, Bhuvaneswari T, Panigrahi A, Ravichandran P (2018) Pythium insidiosum as a new opportunistic fungal pathogen for Pacific white shrimp, Litopenaeus vannamei. Indian J Geomarine Sci 47:1036–1041Google Scholar
- Panigrahi A, Saranya C, Sundaram M, Vinoth Kannan SR, Das Rasmi R, Satish Kumar R, Rajesh P, Otta SK (2018) Carbon: nitrogen (C:N) ratio level variation influences microbial community of the system and growth as well as immunity of shrimp (Litopenaeus vannamei) in biofloc based culture system. Fish Shellfish Immunol 81:329–337CrossRefGoogle Scholar
- Panigrahi A, Sundaram M, Sarany C, Rajasekar S, Syama Dayal J, Gopal C (2019a) Effect of carbon and nitrogen ratio (C: N) manipulation on the production performance and immunity of Pacific white shrimp Litopenaeus vannamei (Boone, 1931) in a biofloc-based rearing system. Aquac Res 50(1):29–41CrossRefGoogle Scholar
- Panigrahi A, Sundaram M, Saranya C, Kumar RS, Dayal JS, Saraswathy R, Otta SK, Anand PS, Rekha PN, Gopal C (2019b) Influence of differential protein levels of feed on production performance and immune response of pacific white leg shrimp in a biofloc–based system. Aquacult 503:118–127CrossRefGoogle Scholar
- Panigrahi A, Sundaram M, Saranya C, Swain S, Das RR, Dayal JS (2019c) Carbohydrate sources deferentially influence growth performances, microbial dynamics and immunomodulation in Pacific white shrimp (Litopenaeus vannamei) under biofloc system. Fish Shellfish Immunol 86:1207–1216CrossRefGoogle Scholar
- Suryakumar B, Avnimelech Y (2017) Adapting biofloc technology for use in small-scale ponds with vertical substrate. World Aquacult 55Google Scholar
- Waite R, Beveridge M, Brummett R, Castine S, Chaiyawannakarn N, Kaushik S, Mungkung R, Nawapakpilai S, Phillips M (2014) Improving productivity and environmental performance of aquaculture. WorldFish:1–60Google Scholar
- Yuvaraj N, Arul V (2014) Preliminary screening of anti-biofilm, anti-larval settlement and cytotoxic potential of seaweeds and seagrasses collected from Pondicherry and Rameshwaram coastal line, India. World J Fish Mar Sci 6(2):169–175Google Scholar