Streptococcosis in Oreochromis sp.: is feed-based biofilm vaccine of Streptococcus agalactiae effective?

  • M. Kahieshesfandiari
  • M. Y. SabriEmail author
  • M. Y. Ina-salwany
  • M. D. Hassan
  • O. Noraini
  • A. A. Ajadi
  • A. I. Isiaku


This study was carried out to determine the potential of a biofilm vaccine of Streptococcus agalactiae in protecting tilapia from streptococcocal infection. The tilapia were vaccinated orally using the biofilm vaccine with 1091010 CFU/g (FBV) incorporated in feed and killed whole-cell vaccine (FCV) of S. agalactiae also prepared in feed, and the unvaccinated control group (Cx) was fed with standard feed formulation. Throughout the study, serum, mucus and gut lavage fluid were collected to evaluate the antibody responses among the groups. The results showed that tilapia vaccinated with FBV exhibited significantly (p < 0.05) stronger immune response and high protection with the relative percentage survival (RPS) of more than 70% (85%) compared with the FCV group, which was only 56%. None of the tilapia in the Cx group survived (0%) post-challenge. Thus, the study revealed that the feed-based biofilm vaccine (FBV) significantly confers the immune protection against the high-dose challenge of S. agalactiae by secretion of the protective immunity in the mucosal and humoral in tilapia.


Biofilm Streptococcus agalactiae Oreochromis sp. Oral vaccination 



We would like to thank the farmer involved in this study and staff of Aquaculture Experimental Station, Faculty of Agriculture, UPM, Puchong, Selangor, for their excellent technical assistance.

Funding information

This project was funded by the Ministry of Science, Technology and Innovation, Malaysia (MOSTI) under the vote no. 5450652.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable institutional guidelines for the care and use of animals were followed by the authors.


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • M. Kahieshesfandiari
    • 1
  • M. Y. Sabri
    • 2
    Email author
  • M. Y. Ina-salwany
    • 1
  • M. D. Hassan
    • 3
  • O. Noraini
    • 2
  • A. A. Ajadi
    • 2
  • A. I. Isiaku
    • 2
  1. 1.Department of Aquaculture, Faculty of AgricultureUniversiti Putra MalaysiaSerdangMalaysia
  2. 2.Deparment of Veterinary Pathology & Microbiology, Faculty of Veterinary MedicineUniversiti Putra MalaysiaSerdangMalaysia
  3. 3.Department of Veterinary Clinical, Faculty of Veterinary MedicineUniversiti Putra MalaysiaSerdangMalaysia

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