Aquaculture International

, Volume 27, Issue 1, pp 279–286 | Cite as

Betanodavirus-associated mortality in Asian seabass (Lates calcarifer, Bloch) cultured in indoor tanks and sea cages

  • S. R. Krupesha Sharma
  • M. A. Pradeep
  • P. N. Dube
  • T. V. Arun Kumar
  • Raj Kumar
  • T. Raja Swaminathan


Sea cage farming of Asian seabass is a prospective aquaculture activity in India. Reports from India on betanodavirus-induced mortality in marine fish culture are not many. An outbreak of viral encephalopathy and retinopathy associated with red-spotted grouper nervous necrosis virus (RGNNV) genotype of betanodavirus in Asian seabass juveniles reared in indoor tanks and sea cages is described in the present paper. Mortality was less severe in cages when compared to tanks. Sequence analysis of the coat protein gene indicated that the virus belonged to RGNNV genotype but found to be distinct from other reported RGNNV isolates. Virus was able to induce cytopathic effects in fish cell lines. Light microscopic features of the disease are also described.


Asian seabass Betanodavirus RT-PCR Sequencing Cell culture Cage culture 



The authors thank Dr. A. Gopalakrishnan, Director, and Dr. P. Vijayagopal, Head of the Marine Biotechnology Division, CMFRI, for the facilities provided.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed by the authors.


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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Central Marine Fisheries Research InstituteKochiIndia
  2. 2.Research Centre of CMFRIKarwarIndia
  3. 3.PMFGR Centre of National Bureau of Fish Genetic ResourcesKochiIndia

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