Aquaculture International

, Volume 27, Issue 1, pp 239–260 | Cite as

Effects of autochthonous probiotics, isolated from Tor grypus (Karaman, 1971) intestine and Lactobacillus casei (PTCC 1608) on expression of immune-related genes

  • Takavar MohammadianEmail author
  • Mojtaba Alishahi
  • Mohammad Reza Tabandeh
  • Abdolhossein Jangaran Nejad
  • Esmaeil Karami
  • Mojtaba Zarea


In this study, Lactobacillus plantarum subsp. plantarum and L. delbrukei subsp. bulgaricus were used as autochthonous food supplementation for gene expression and hematological investigation. Then, 480 fish (BW, 45 ± 10 g) were randomly divided into four groups (three replications) and were fed with diet containing 5 × 107 CFU g−1 of each isolated LAB and L. casei (PTCC 1608) and a control diet with no bacteria. Feeding trail was conducted for 60 days, following a change to the non-supplemented feed until 15 days (day 75). Samples from blood and head kidney at days 0, 60, and 75 were taken. The in vitro examination showed that L. plantarum had high antimicrobial activity against the Aeromonas hydrophila and Yesinia ruckeri. Results of this study showed that feeding of T. grypus with L. plantarum supplementation diet led to increase Hb and WBC, and induce TNF-α, IL-8, and IL-1β expressions in the head kidney rather than control. Results suggested that L. plantarum can be applied as a dietary supplement to enhance the immunity system of T. grypus.


Probiotics TNF-α IL-8 IL-1β Tor grypus 


Funding information

This work was funded by a grant from Shahid Chamran University of Ahvaz Research Council (Grant Nos. 27176, 1393.3.2).

Compliance with ethical standards

All institutional and national guidelines for the care and use of laboratory animals were followed.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Takavar Mohammadian
    • 1
    Email author
  • Mojtaba Alishahi
    • 1
  • Mohammad Reza Tabandeh
    • 2
  • Abdolhossein Jangaran Nejad
    • 1
  • Esmaeil Karami
    • 1
  • Mojtaba Zarea
    • 3
  1. 1.Department of Clinical Sciences, Faculty of Veterinary MedicineShahid Chamran University of AhvazAhvazIran
  2. 2.Department of Biochemistry and Molecular Biology, Faculty of Veterinary MedicineShahid Chamran University of AhvazAhvazIran
  3. 3.Young Researchers and Elites ClubAhvazIran

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